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An alkaline β-mannanase was purified to homogeneity from a culture broth of alkaliphilic Bacillus sp. N16-5. The enzyme had optimum activity at pH 9.5 and 70°C. It was composed of a single polypeptide chain with a molecular weight of 55 kDa deduced from SDS-PAGE, and its isoelectric point was around pH 4.3. The enzyme efficiently hydrolyzed galactomannan(More)
Bacterial diversity associated with Baer Soda Lake in Inner Mongolia of China was investigated using a culture-independent method. Bacterial 16S rRNA gene libraries were generated using bacterial oligonucleotide primers, and 16S rRNA gene sequences of 58 clones were analyzed phylogenetically. The library was dominated by 16S rDNAs of Gram-negative bacteria(More)
A novel haloalkaliphilic archaeon, strain DZ-1T, was isolated from a soda lake in Xinjiang, China. The taxonomy of strain DZ-1T was studied by polyphasic methods. According to 16S rRNA gene sequence similarity, strain DZ-1T was phylogenetically related to Halorubrum tibetense (97.5 %), Halorubrum vacuolatum (95.7 %) and Halorubrum saccharovorum (95.9 %).(More)
A novel alkaliphilic and moderately halophilic gram-positive coccus, designated strain T8T, was isolated from Baer Soda Lake in Inner Mongolia Autonomous Region, China. Strain T8T grew in the presence of 0-25% (w/v) NaCl and at pH 6.5-11.5, with optimum growth at 10% (w/v) NaCl and pH 9.0. It grew at 10.0-46.0 degrees C, with an optimum growth temperature(More)
Three novel isolates of haloalkaliphilic archaea, strains IHC-005T, IHC-010, and N-1311T, from soda lakes in Inner Mongolia, China, were characterized to elucidate their taxonomic positions. The three strains were aerobic, Gram-negative chemoorganotrophs growing optimally at 37–45°C, pH 9.0–9.5, and 15–20% NaCl. Cells of strains IHC-005T/IHC-010 were motile(More)
A Gram-positive, rod-shaped, spore-forming and moderately halophilic bacterium (strain 8-1(T)) was isolated from a sediment sample of a neutral salt lake in Xin-Jiang, China. The strain grew optimally at 30 degrees C, pH 7.0-7.5 and 8-12 % (w/v) NaCl. The major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0). The predominant(More)
A Gram-positive, halophilic bacterium was isolated from a sediment sample from Ai-Ding salt lake in China. The isolate, designated strain 17-5(T), grew at salinities of 8-33 % (w/v) NaCl (optimally at 12 %, w/v). The genomic DNA G+C content of strain 17-5(T) was 48.1 mol%. The predominant isoprenoid quinone was MK-7(H(2)) and the cell-wall peptidoglycan(More)
Two novel psychrophilic bacterial strains (ZF-6(T) and ZF-8(T)) were isolated from the China No. 1 glacier. Polyphasic taxonomy using physiological and biochemical properties and phylogenetic analysis based on 18S rRNA gene sequences showed that the two isolates belonged to the genus Flavobacterium, and that they were distinct from each other and also from(More)
The extremely halophilic archaeon ZP-6 was isolated from Ai-Ding salt lake in Xinjiang Uighur Autonomous Region of the People's Republic of China. Based on its physiological properties, 16S rDNA sequence, and DNA–DNA homology with known haloarchaea, the isolate was tentatively identified as a Halobacterium sp. An acetoacetyl-CoA thiolase was purified and(More)
The genome of Thermoanaerobacter tengcongensis is estimated to encode 2588 theoretical proteins. In this study, we have vitalized approximately 46% of the theoretical proteome experimentally using a proteomic strategy that combines three different methods, shotgun digestion plus high-performance liquid chromatography (HPLC) with ion-trap tandem mass(More)