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Bovine lactoferrin (LTF) is a multifunctional small glycoprotein found in milk acting mainly as a defense factor in the mammary gland. Many polymorphisms have been found in the bovine LTF gene but almost none were considered as genetic markers of production traits in dairy cattle. In this study, the promoter fragment of LTF gene containing mutation (G/C) in(More)
Prolactin plays an important regulatory function in mammary gland development, milk secretion, and expression of milk protein genes. Hence the PRL gene is a potential quantitative trait locus and genetic marker of production traits in dairy cattle. We analysed the sequence of the PRL gene to investigate whether mutations in this sequence might be(More)
A new single nucleotide polymorphism was revealed using PCR–SSCP and sequencing methods within the bovine prolactin distal promoter region described as a functional enhancer. The A→G transition at position −1043 abolishes the recognition site for Hsp92II restriction endonuclease, allowing for PCR–RFLP genotyping. The application of real-time PCR revealed(More)
Milk protein genes expression in cows' mammary epithelial cells is regulated mostly by the action of prolactin mediated through the STAT5A transcription factor. The STAT5A gene is a potential quantitative trait locus (QTL) and genetic marker of production traits in dairy cattle. The sequence of the bovine STAT5A gene was analysed in this study to(More)
SNiPORK is an oligonucleotide microarray based on the arrayed primer extension (APEX) technique, allowing genotyping of single nucleotide polymorphisms (SNPs) in genes of interest for pork yield and quality traits. APEX consists of a sequencing reaction primed by an oligonucleotide anchored with its 5' end to a glass slide and terminating one nucleotide(More)
Real-time quantitative reverse transcription PCR (qRT-PCR) is the method of choice to investigate the alterations in gene expression involved with BLV pathogenesis. However, the reliability of qRT-PCR data critically depends on proper normalization to a set of stably expressed reference genes. The aim of the study was to validate the expression stability of(More)
In marker-assisted selection (MAS) of dairy cattle certain genes are proposed as potential candidates associated with dairy performance traits. Among different candidates, prolactin receptor gene (PRLR) seems to be promising because of its crucial role in transmitting signal from lactogenic hormones to milk protein gene promoters. In this study nine PCR(More)
It is currently debated whether identification of ESR (estrogen receptor) genotypes should be introduced into breeding programs of Large White pigs. The aim of this study was to evaluate the possible relations between ESR/Ava I polymorphism and carcass performance traits in Polish Large White boars. We examined 103 boars originating from one herd in NE(More)
An oligonucleotide microarray-which allows for parallel genotyping of many SNPs in genes involved in cow milk protein biosynthesis-was used to identify which of the 16 candidate SNPs are associated with milk performance traits in Holstein cows. Four hundred cows were genotyped by the developed and validated microarray. Significant associations were found(More)
The carcasses of yellow-fat rabbits may be attractive to modern consumers, because they have a relatively high content of biologically active compounds. One of the main candidate genes associated with the yellow-fat trait is β-carotene 9′,10′-oxygenase (BCO2). This study is the first report of the novel AAT-deletion mutation at codon 248 of the BCO2 gene,(More)