Pavlina D. Konstantinova

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RNA interference (RNAi) is a powerful approach to inhibit human immunodeficiency virus type 1 (HIV-1) replication. However, HIV-1 can escape from RNAi-mediated antiviral therapy by selection of mutations in the targeted sequence. To prevent viral escape, multiple small interfering RNAs (siRNAs) against conserved viral sequences should be combined. Ideally,(More)
This paper compares two algorithms for Multiple Target Tracking (MTT), using Global Nearest Neighbor (GNN) and Suboptimal Nearest Neighbor (SNN) approach respectively. For both algorithms the observations are divided in clusters to reduce computational efforts. For each cluster the assignment problem is solved by using Munkres algorithm or according SNN(More)
– In this paper we consider and analyze the behavior of two combinational rules for temporal (sequential) attribute data fusion for target type estimation. Our comparative analysis is based on Dempster's fusion rule proposed in Dempster-Shafer Theory (DST) and on the Proportional Conflict Redistribution rule no. 5 (PCR5) recently proposed in(More)
ABC transporters export clinically-relevant drugs and their over-expression causes multidrug resistance. In order to knock-down ABC transporters, ABCC1 and ABCC2, 13 shRNAs were developed. Four shRNA candidates were tested in vivo using self-complementary adeno-associated virus serotype 8. A strong, specific knock-down of Abbc2 was observed in mice liver,(More)
RNA interference (RNAi) has been successfully employed for specific inhibition of gene expression; however, safety and delivery of RNAi remain critical issues. We investigated the combinatorial use of RNAi and U1 interference (U1i). U1i is a gene-silencing technique that acts on the pre-mRNA by preventing polyadenylation. RNAi and U1i have distinct(More)
Overexpression of short hairpin RNA (shRNA) often causes cytotoxicity and using microRNA (miRNA) scaffolds can circumvent this problem. In this study, identically predicted small interfering RNA (siRNA) sequences targeting apolipoprotein B100 (siApoB) were embedded in shRNA (shApoB) or miRNA (miApoB) scaffolds and a direct comparison of the processing and(More)
Potent antiviral RNAi can be induced by intracellular expression of short hairpin RNAs (shRNAs) and artificial microRNAs (miRNAs). Expression of shRNA and miRNA results in target mRNA degradation (perfect base pairing) or translational repression (partial base pairing). Although efficient inhibition can be obtained, error-prone viruses such as human(More)
Recombination due to template switching during reverse transcription is a major source of genetic variability in retroviruses. In the present study we forced a recombination event in human immunodeficiency virus type 1 (HIV-1) by electroporation of T cells with DNA from a molecular HIV-1 clone that has a 300 bp long hairpin structure in the Nef gene(More)
In this paper the effectiveness of two Data Association algorithms for Multiple Target Tracking (MTT) based on Global Nearest Neighbor approach are compared. As the time for assignment problem solution increases nonlinearly depending on the problem size, it is useful to divide the whole scenario on small groups of targets called clusters. For each cluster(More)
BACKGROUND Human immunodeficiency virus type 1 (HIV-1) can be inhibited by means of RNA silencing or interference (RNAi) using synthetic short interfering RNAs (siRNAs) or gene constructs encoding short hairpin RNAs (shRNAs) or long hairpin RNAs (lhRNAs). The use of siRNA and shRNA as antiviral therapeutic is limited because of the emergence of viral escape(More)