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Tubulin, the major constituent protein of microtubules, is a heterodimer of alpha and beta subunits. Both alpha and beta exist in multiple isotypic forms. It is not clear if different isotypes perform different functions. In order to approach this question, we have made a monoclonal antibody specific for the beta III isotype of tubulin. This particular(More)
Tubulin, the 100-kDa subunit protein of microtubules, is a heterodimer of two 50-kDa subunits, alpha and beta. Both alpha and beta subunits exist as numerous isotypic forms. There are four isotypes of beta-tubulin in bovine brain tubulin preparations; their designations and relative abundances in these preparations are as follows: beta I, 3%; beta II, 58%;(More)
Piétrain boars of different ryanodine receptor (RYR1) genotypes (NN, Nn and nn, three boars each) were mated with approximately 10 Czech Large White x Czech Landrace sows (genotype NN) each to produce one litter per sow. The progeny of nn boars had a significantly higher individual weaning weight (7.31 kg versus 6.86 kg) and average daily gain from birth to(More)
BACKGROUND Some study reported circadian occurence of sudden cardiac death, ventricle ectopic activity, acute coronary syndromes and heart rate variability. Heart rate turbulence (HRT) is one of a new markers of noninvasive stratification of sudden cardiac death. METHODS AND RESULTS We have evaluated HRT in 120 consecutive patiens post myocardial(More)
BACKGROUND The presence of a microvolt T wave alternans (MTWA) is linked with increased risk of malignant arrhythmias and overall mortality. The most common method used for MTWA detection is a bicycle exercise test (BET). Method has still several limitations. AIM To confirm that comparable MTWA results may be obtained by atrial and ventricular pacing(More)
Some studies have demonstrated circadian incidence of sudden cardiac death (SCD), ventricular ectopies, acute coronary syndromes and heart rate variability. One of new parameters applied in non-invasive stratification of sudden cardiac death is heart rate turbulence (HRT). Detection of circadian oscillations in HRT and optimised measurement of HRT can(More)
Derivatives of 6-benzyl-1,3-benzodioxole are known to bind to tubulin and inhibit tubulin polymerization. For a better understanding of the mechanism of action of the 6-benzyl-1,3-benzodioxole derivatives, we have examined their effect on the alkylation of tubulin sulfhydryls by iodo[14C]acetamide and N,N'-ethylene(bis)iodoacetamide. We have found that the(More)
Beta 1 and beta 2 are the designations given to two forms of beta-tubulin that have different electrophoretic mobilities on discontinuous polyacrylamide gels in the presence of sodium dodecyl sulfate [Little, M. (1979) FEBS Lett. 108, 283-286]. Beta 1 and beta 2 constitute respectively 75% and 25% of the total beta-tubulin in bovine brain. Although beta 1(More)
2-(4-Methyl-1-piperazinylmethyl) acrylophenone dihydrochloride (MPMAP) is a novel inhibitor of microtubule assembly in vitro and in vivo whose molecular mechanism of action has not been investigated (M. L. Mallevais, A. Delacourte, I. Lesieur, D. Lesieur, M. Cazin, C. Brunet, and M. Luyckx (1984) Biochimie 66, 477-482). We have examined the effect of MPMAP(More)