Pavagada Krishnamurthy Maheshwar

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A specific and sensitive reverse transcriptase-nested polymerase chain reaction assay (RT-nPCR) was developed for the detection of Citrus tristeza virus (CTV) from naturally infected citrus samples. Two sets of primer pairs were designed by alignment of nucleotide sequences available in GenBank database for different genotypes of CTV. RT-nPCR reaction(More)
Plant pathogens like ‘Ca. Liberibacter’, phytoplasma, viruses and viroids cause diseases to almost all economically important plants. A simple and fast sap-mediated polymerase chain reaction (PCR) method for the detection of these pathogens infecting various plant hosts is described in the present study. Sap from selected plants was drawn aseptically on(More)
‘ Candidatus Liberibacter asiaticus’ was detected in Citrus decumana displaying symptoms of greening disease by direct polymerase chain reaction using universal primers specific to ribosomal protein gene of liberibacter. 100% Sequence identities were found with isolates of ‘Ca. L. asiaticus'—isolated from mandarins from Karnataka, India and more than 96%(More)
Association of Chrysanthemum stunt viroid (CSVd) and Chrysanthemum chlorotic mottle viroid (CChMVd) with the Chrysanthemum plants exhibiting severe stunting, distinct yellow leaf mottling, and chlorosis was detected in the main chrysanthemum-growing regions of India. Sequence analysis of 90 cDNA clones obtained for CSVd and CChMVd, representing the(More)
Contamination of paddy seeds (rice with husk) by Fusarium species can cause spoilage and subsequent production of mycotoxins, especially fumonisins that affect human and animal health. A mycological study was conducted to evaluate the natural occurrence of fumonisin B1 produced by Fusarium proliferatum on paddy grown in different geographic regions of(More)
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