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Autotetraploid Coffea canephora and Auto-Alloctaploid Coffea arabica From In Vitro Chromosome Set Doubling: New Germplasms for Coffea
TLDR
The CSD procedure, exploring the CAS proliferation and ISE pathway, promoted whole-genome duplication and resulted in a relatively high number of solid polyploids of both Coffea species and potentially other shrubbery and woody species.
The contribution of cytogenetics and flow cytometry for understanding the karyotype evolution in three Dorstenia (Linnaeus, 1753) species (Moraceae)
TLDR
The karyological analysis suggests a polyploid origin of the Dorstenia species studied here, and structural rearrangements are related to the karyotype variations among the three species.
Indirect somatic embryogenesis in Coffea with different ploidy levels: a revisiting and updating study
TLDR
The findings provide the basis for performing other approaches considering the ploidy level, epigenetic and physiological backgrounds and contributed for understanding about the consequences of polyploidy.
From chromosome doubling to DNA sequence changes: outcomes of an improved in vitro procedure developed for allotriploid “Híbrido de Timor” (Coffea arabica L. × Coffea canephora Pierre ex A. Froehner)
TLDR
Besides confirmation of endomitosis induction through the obtained hexaploids, SSR markers revealed that the FEC/colchicine strategy also resulted in loss of allelic diversity in 39 regenerated HT plantlets, demonstrating its genotoxic effect.
Global 5-methylcytosine and physiological changes are triggers of indirect somatic embryogenesis in Coffea canephora
TLDR
The competence acquisition, determination, and cell differentiation steps were marked by increases in mean values of 5-mC%, IAA and ABA, and decreases in ACC and Spd, evincing that these changes are the triggers for regeneration and maturation of somatic embryos.
First karyotype description and nuclear 2C value for Myrsine (Primulaceae): comparing three species.
TLDR
The first description of the Myrsine karyotype has been presented, as well as the nuclear 2C value and chromosome morphometry, and the procedures can be applied for future investigations to better understand its effects on the differential spatial occupation abilities of the species in Brazilian Atlantic Forest.
First karyotype description and nuclear 2C value for Myrsine (Primulaceae): comparing three species
TLDR
The first description of the Myrsine karyotype has been presented, as well as the nuclear 2C value and chromosome morphometry, and the procedures can be applied for future investigations to better understand its effects on the differential spatial occupation abilities shown by the species in Brazilian Atlantic Forest.