Paulo Alves Garcia

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Electroporation is commonly used to deliver molecules such as drugs, proteins, and/or DNA into cells, but the mechanism remains poorly understood. In this work a rapid microfluidic assay was developed to determine the critical electric field threshold required for inducing bacterial electroporation. The microfluidic device was designed to have a bilaterally(More)
We developed protocols for, and demonstrated successful transfection of, the free-living kinetoplastid flagellate Parabodo caudatus with three plasmids carrying a fluorescence reporter gene (pEF-GFP with the EF1 alpha promoter, pUB-GFP with Ubiquitin C promoter, and pEYFP-Mitotrap with CMV promoter). We evaluated three electroporation approaches: (1) a(More)
The wireless networks have been the object of many studies and analyzes of current technology industry, providing not only communication with mobility to end users, but also incorporating new applications. One such technology is known as Wireless Fidelity (Wi-Fi) 802.11. This paper aims to present the behavior of the propagation of electromagnetic waves(More)
This paper presents the results of a PhD thesis research, with an innovative proposal for the development of a Technical Laboratory Disciplines in Electrical and Computing Engineering Schools. The presented system allows that the Teacher-Student interaction and the Teacher support be improved by using IT and Internet Communication. This proposal is an(More)
Synthetic biology holds great potential for addressing pressing challenges for mankind and our planet. One technical challenge in tapping into the full potential of synthetic biology is the low efficiency and low throughput of genetic transformation for many types of cells. In this paper, we discuss a novel microfluidic system for improving bacterial(More)
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