Paula J. M. van Kleeff

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Protein phosphorylation is essential for many aspects of plant growth and development. To fully modulate the activity of specific proteins after phosphorylation, interaction with members of the 14-3-3 family is necessary. 14-3-3 Proteins are important for many processes because they “assist” a wide range of target proteins with divergent functions. In this(More)
Gene redundancy is a common cause for the absence of observable phenotypic alterations in single mutant plants. Gene redundancy is a phenomenon in which family members of the mutated gene compensate for its loss. In yeast, one protein family in which gene redundancy has been observed is 14-3-3. In Arabidopsis, single mutant phenotypes have been found for(More)
A lack of phenotype in a loss-of-function mutant could be caused by genetic redundancy in which a closely related gene compensates for the loss of the mutant allele(s). In this Chapter, we show the involvement of six non-epsilon Arabidopsis 14-3-3 members (three most similar pairs) in root and shoot growth/ development. The root phenotyping on all 24 14-3-3(More)
The plant's response to abiotic stress involves protein phosphorylation cascades. The 14-3-3 protein family binds to phosphorylated proteins thereby situating itself in numerous signal transduction cascades making it highly likely that 14-3-3's are involved in stress adaptation. In this study we used six members (three closely related pairs) of the(More)
Plant photosynthesis produces carbohydrates necessary for growth and development, the main photosynthetic product being sucrose. Sucrose is transported from the photosynthetic areas (source) of the plant to non-photosynthetic areas (sink). Within sink tissue sucrose is converted to hexoses by two pathways in which: 1) sucrose is reversibly converted to(More)
Posttranslational protein modifications, in particular reversible protein phosphorylation, are important regulatory mechanisms involved in cellular signaling transduction pathways. Thousands of human proteins are phosphorylatable and the tight regulation of phosphorylation states is crucial for cell maintenance and development. Protein phosphorylation(More)
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