Paula HO Lima

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BACKGROUND Diabetes in pregnant women is associated with an increased risk of maternal and neonatal morbidity and remains a significant medical challenge. Diabetes during pregnancy may be divided into clinical diabetes and gestational diabetes. Experimental models are developed with the purpose of enhancing understanding of the pathophysiological mechanisms(More)
PURPOSE To investigate the effects of insulin, epidermal growth factor (EGF), and the corneal storage media--DexSol--at 24 and 48 hours on DNA synthesis in confluent primary cultures of bovine corneal endothelial cells. METHODS Flow cytometry was used to measure changes in DNA synthesis. This technique allows a large number of cells to be counted and(More)
The effects of gentamicin, tobramycin and the fluorquinolone, ofloxacin on corneal epithelial wound healing were determined using an in vitro model of wound healing. Wounds were created in rabbit corneal epithelial cultures which were then exposed to various concentrations of the antibiotics. Effects on wound healing were measured by comparing migration(More)
OBJECTIVE Experimental studies have shown that exposure to cigarette smoke has negative effects on lipid metabolism and oxidative stress status. Cigarette smoke exposure in nonpregnant and pregnant rats causes significant genotoxicity (DNA damage). However, no previous studies have directly evaluated the effects of obesity or the association between obesity(More)
PURPOSE Differential display analysis was used to look for gender differences in lacrimal gland gene expression. The expression of a female-specific mouse lacrimal gland mRNA that encoded pancreatic lipase-related protein 1 (PLRP1) was identified and characterized. METHODS Differential display analysis of the exorbital lacrimal glands of male and female(More)
BACKGROUND The purpose of this study was to evaluate the effects of cigarette smoke exposure before pregnancy on diabetic rats and their offspring development. METHODS Diabetes was induced by streptozotocin and cigarette smoke exposure was conducted by mainstream smoke generated by a mechanical smoking device and delivered into a chamber. Diabetic female(More)
Because of its usefulness in treating facial rosacea, metronidazole may also be an effective treatment for ocular rosacea. The toxicity of metronidazole on the corneal epithelium has not previously been reported. In this study, the effects of metronidazole on rabbit corneal epithelium in vitro were evaluated. Epithelial cell cultures were exposed to various(More)
Using Dispase-treated rat corneas, primary cultures of corneal epithelium have been established which are free of contaminating cell types. Cultures were maintained for periods up to 36 days and were monitored with morphological and electrophysiological methods. Phase and scanning electron microscopy revealed a mosaic of polygonal epithelial cells which(More)
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