Paul R Burton

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Axonal extensions of neurons show microtubules (MTs) with a uniform polarity indicating that they originate at or near the perikaryon. To obtain information on the polarity of dendritic MTs, a polarity assay was used to examine MTs in dendrites of interneurons (mitral cells) of the olfactory pathway of the bullfrog. The assay involved incubating pieces of(More)
Pieces of olfactory nerve of the bullfrog were extracted in a tubulin assembly buffer medium containing detergents. With incubation at 37 degrees C in such medium containing soluble tubulin, ribbons of protofilaments are formed on the surfaces of microtubules, with the ribbons curving in a clockwise or counterclockwise direction. The direction of hooking(More)
The ultrastructure of the accessory olfactory bulb (AOB) of the bullfrog tadpole and adult was examined, and the main difference between tadpole and adult is that the latter is more compact and shows more synapses. Except for vomeronasal (VMN) glomeruli, the AOB is not highly organized, with mitral cell neurons scattered throughout the neuropil. VMN axon(More)
The substructure and distribution of luminal material in microtubules of olfactory axons were studied in the bullfrog, Rana catesbeiana. By using numerous fixation methods, with and without osmium tetroxide, the luminal component was shown not to be an artifact of fixation. The material consists of globular elements 4-5 nm in diameter loosely arranged(More)
The ciliated dendritic bulb of the olfactory neuron of the bullfrog was studied with the electron microscope, with emphasis on microtubular elements. Methods used included various fixation procedures with and without detergent extraction, serial sectioning, microtubule polarity assays, and an assay to demonstrate F-actin. Structural continuity exists, via(More)
The distribution of primary olfactory and vomeronasal (VMN) glomeruli is demonstrated in the forebrain of the larval (stage III tadpole) and adult bullfrog. Olfactory glomeruli are massed at the anterior end of the olfactory bulb; farther posterior, they are located at the periphery and then become distributed in a superficial layer all along the ventral(More)
The olfactory neuron is specialized along its length into highly determined morphological regions. These regions include the dendritic cilia, dendritic vesicle, dendritic shaft proper, perikaryon, axon, zone of transition where the axon widens as it approaches its termination, and the axon terminal. Except for the zone of transition and the terminal,(More)
Intra-ocular pressure (IOP) was measured immediately after normal subjects were woken from at least 5 hours sleep. Measurements were made at approximately 15 s intervals, for about 20 minutes. The IOP of all 14 subjects was elevated after sleep and returned to baseline levels with a time course which was approximately exponential; the longest time constant(More)
In the present study, the structural and functional role of smooth endoplasmic reticulum was investigated in bullfrog olfactory axon terminals. Structural evidence obtained from this study indicated that this vesiculotubular organelle becomes a more elaborate network of anastomosing tubules near the nerve terminal, located in the olfactory lobe of frog(More)