Learn More
Mutations in the adenomatous polyposis coli (APC) gene are linked to polyp formation in familial and sporadic colon cancer, but the functions of the protein are not known. We show that APC protein localizes mainly to clusters of puncta near the ends of microtubules that extend into actively migrating regions of epithelial cell membranes. This subcellular(More)
The regulation of cell growth and survival can be sub-verted by a variety of genetic defects that alter transcrip-tional programs normally responsible for controlling cell number. High throughput analysis of these gene expression patterns should ultimately lead to the identification of minimal expression profiles that will serve as common denominators in(More)
Mutations in the adenomatous polyposis coli (APC) tumor suppressor gene are linked to both familial and sporadic human colon cancer. So far, a clear biological function for the APC gene product has not been determined. We assayed the activity of APC in the early Xenopus embryo, which has been established as a good model for the analysis of the signaling(More)
Background: Inactivation of the adenomatous polyposis coli (APC) tumor suppressor protein is responsible for both inherited and sporadic forms of colon cancer. Growth control by APC may relate to its ability to downregulate β-catenin post-translationally. In cancer, mutations in APC ablate its ability to regulate β-catenin, and mutations in β-catenin(More)
is Wnt-1 itself, which promotes tumorigenesis when ec-1 DNA Way topically expressed in the murine mammary gland. As South San Francisco, California 94080 a secreted ligand, Wnt activates frizzled, a cell surface receptor that engages the intracellular protein dishev-eled that in turn interferes with glycogen synthase kinase The targeted destruction of(More)
Aberrant regulation of the Wnt signalling pathway has emerged as a prevalent theme in cancer biology. This chapter summarizes the research that provides a proof of concept for inhibiting Wnt signalling in cancer, the potential means by which this could be achieved, and some recent advances towards this goal. A brief discussion of molecular diagnostics and(More)
Capillaries in the brain are especially selective in determining which blood-borne components gain access to neurons. The structural elements of this blood-brain barrier (BBB) reside at the tight junction, an intercellular protein complex that welds together adjacent endothelial cell membranes in the microvasculature. In this issue, Liebner et al. (Liebner,(More)
Canonical Wnt signaling is controlled intracellularly by the level of β-catenin protein, which is dependent on Axin scaffolding of a complex that phosphorylates β-catenin to target it for ubiquitylation and proteasomal degradation. This function of Axin is counteracted through relocalization of Axin protein to the Wnt receptor complex to allow for(More)