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We have investigated the requirement for Ca 2 1 in the fusion and content mixing of rat hepatocyte late endosomes and lysosomes in a cell-free system. Fusion to form hybrid organelles was inhibited by 1,2bis(2-aminophenoxy) ethane-N,N,N 9 ,N 9 -tetraacetic acid (BAPTA), but not by EGTA, and this inhibition was reversed by adding additional Ca 2 1 . Fusion(More)
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