Paul Jouan

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The action of estradiol-17 beta (E2) on thymidine kinase (TK) activity was studied in uteri from immature female rats. It was demonstrated that a single injection of E2 highly stimulated the enzyme activity which reached its maximum level 24 h after hormone administration. Physiological amounts of E2 were efficient and changes in TK activity were observed(More)
In anterior pituitaries from male rats, it appeared that 5 alpha-androstane-3 beta,17 beta-diol was quickly metabolized into 5 alpha-androstane-3 beta,6 alpha-17 beta-triol and 5 alpha-androstane-3 beta,7 alpha,17 beta-triol by action of 6 alpha- and 7 alpha-hydroxylases. Hydroxysteroid hydroxylases were located in endoplasmic reticulum and were dependent(More)
The presence of thymidine phosphorylase in human healthy, adenomatous and cancerous prostate was demonstrated. The enzyme was responsible for the cleavage and synthesis of thymidine and for the transfer of deoxyribose from one deoxyribonucleoside to a pyrimidic base. The enzyme from normal and adenomatous prostate was retained on DEAE-Sephadex gel. In PC-3(More)
Mammary tumors were promoted in male rats of the Wistar WAG strain by continuous and simultaneous administration of 17 beta-estradiol and progesterone. Tumor induction and growth were dependent on estradiol and on progesterone. Their histological features were comparable with those of human breast cancers. Hormone receptors were present in tumor cells.(More)
The binding of [3H]5 alpha-androstane-3 beta, 17 beta-diol (3 beta-Adiol) in the pituitary cytosol of prepubertal male rats was studied. The following criteria indicated that 3 beta-Adiol was bound to specific proteins and, in all probability, to the estrogen receptor. 1) The binding was of relatively high affinity and low capacity. 2) The complex was(More)
Acetabular loosenings with severe bone loss require a large amount of graft material to fill the defect. Although all donors are systematically subjected to serologic screening, bone allografts that are chemically processed, freeze-dried, and radiosterilized ensure a higher level of microbiological safety than radiosterilised deep-frozen allografts. The(More)
The fetal isoenzyme of thymidine kinase was purified to apparent homogeneity from cytosols of rat fetuses liver. A two-step purification including anion exchange chromatography and affinity chromatography was developed. The purified enzyme appears as oligomeric with a relative molecular weight of 71 kDa. In denaturing media its molecular weight was 24 kDa,(More)