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Estrogens and androgens are steroids that act as reproductive hormones in vertebrates. These compounds have also been detected in reef-building corals and other invertebrates, where they are hypothesized to act as bioregulatory molecules. Experiments were conducted using labeled steroid substrates to evaluate metabolism of estrogens and androgens by coral(More)
PURPOSE To investigate the effects of insulin, epidermal growth factor (EGF), and the corneal storage media--DexSol--at 24 and 48 hours on DNA synthesis in confluent primary cultures of bovine corneal endothelial cells. METHODS Flow cytometry was used to measure changes in DNA synthesis. This technique allows a large number of cells to be counted and(More)
17β-Hydroxysteroid dehydrogenase/17-ketosteroid reductase (17HSD/KSR) activity and 17HSD/KSR types 1, 2, 4, and 5 mRNA levels were characterized in ovarian cancer cell lines derived from patients unexposed to radiation or chemotherapy. Activity was at the limit of detection in TOV-112D and TOV-21G cells. Activity in OV-90 was comparable to that in human(More)
The effects of gentamicin, tobramycin and the fluorquinolone, ofloxacin on corneal epithelial wound healing were determined using an in vitro model of wound healing. Wounds were created in rabbit corneal epithelial cultures which were then exposed to various concentrations of the antibiotics. Effects on wound healing were measured by comparing migration(More)
OBJECTIVE Cathepsins B (CB) and D (CD) belong to a family of proteases felt to be important in tumor metastasis and invasion. It has been suggested that both enzymes play a role the progression of epithelial ovarian cancer and they have been investigated as potential biomarkers for ovarian cancer. Our objective was to determine if activity ratios of these(More)
Using Dispase-treated rat corneas, primary cultures of corneal epithelium have been established which are free of contaminating cell types. Cultures were maintained for periods up to 36 days and were monitored with morphological and electrophysiological methods. Phase and scanning electron microscopy revealed a mosaic of polygonal epithelial cells which(More)
PURPOSE Differential display analysis was used to look for gender differences in lacrimal gland gene expression. The expression of a female-specific mouse lacrimal gland mRNA that encoded pancreatic lipase-related protein 1 (PLRP1) was identified and characterized. METHODS Differential display analysis of the exorbital lacrimal glands of male and female(More)
OBJECTIVES Cathepsins B and D belong to a family of proteases involved in tumor invasion and metastasis. As such they may function as biomarkers for the aggressiveness of a given tumor. We examined the enzymatic activity of these proteins as well as the cellular and extracellular distribution of cathepsins B and D. METHODS 39 snap frozen tissue samples(More)
Epidemiologic data suggest a relationship between dietary intake of phytochemicals and a lower incidence of some cancers. Modulation of steroid hormone metabolism has been proposed as a basis for this effect. It has been shown that aromatase, 3beta-hydroxysteroid dehydrogenase and 17beta-hydroxysteroid dehydrogenase (17beta-HSD) are inhibited by the(More)
Steroid metabolism studies have yielded evidence of 17beta-hydroxysteroid dehydrogenase (17beta-HSD) activity in corals. This project was undertaken to clarify whether there are multiple isoforms of 17beta-HSD, whether activity levels vary seasonally, and if zooxanthellae contribute to activity. 17Beta-HSD activity was characterized in zooxanthellate and(More)