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The development of a nanoparticle-based detection methodology for sensitive and specific DNA-based diagnostic applications is described. The technology utilizes gold nanoparticles derivatized with thiol modified oligonucleotides that are designed to bind complementary DNA targets. A glass surface with arrays of immobilized oligonucleotide capture sequences(More)
The application of resonance light scattering (RLS) particles for high-sensitivity detection of DNA hybridization on cDNA microarrays is demonstrated. Arrays composed of approximately 2000 human genes ("targets") were hybridized with colabeled (Cy3 and biotin) human lung cDNA probes at concentrations ranging from 8.3 ng/microL to 16.7 pg/microL. After(More)
The Taylor expansion proposed by Hansen [3] is generalized to degreem and estimates are given for the number of zero entries in the remainder. The expansion is then used to define a Taylor form for the range of a function over an interval and estimates are given for the number of interval variables replaced by real variables due to the special Taylor(More)
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