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Electroporation parameters can be optimized by coupling RC constant values with the amount of electrical power dissipation in the electroporation medium. Electroporation efficiency increases more steeply with power at low power values.
Lecithin and lecithin/cholesterol liposomes formed in aqueous solutions of DNA entrap covalently closed circular, open circular and linear DNA molecules of size up to at least 13 kilobases. The sequestered DNA molecules are efficiently protected against exogenous deoxyribonuclease action although nicking and linearization of circular DNA can be observed.… (More)
This Note questions the safety of crop plants engineered with transgenes coding for the degradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) into its cytotoxic metabolite 2,4-dichlorophenol (2,4-DCP).