• Publications
  • Influence
Involvement and unusual substrate specificity of a prolyl oligopeptidase in class III lanthipeptide maturation.
TLDR
In vitro investigations of the gene cluster from Kribbella flavida, involving reconstitution of the biosynthesis of the new lanthipeptide flavipeptin, proved that a POP-type FlaP protease is responsible for leader removal and revealed that FlaP is specific to the post-translationally modified peptide and can discriminate between N- and C-terminal rings. Expand
Leader peptide-directed processing of labyrinthopeptin A2 precursor peptide by the modifying enzyme LabKC.
TLDR
This work systematically investigated for the first time the importance of the leader peptide in the processing of class III lantibiotics using the example of the labyrinthopeptin A2 precursor peptide and found that a conserved N-terminal hydrophobic patch on a putative helical structure is required for the proper peptide processing by the modifying enzyme LabKC. Expand
Elucidation of sevadicin, a novel non-ribosomal peptide secondary metabolite produced by the honey bee pathogenic bacterium Paenibacillus larvae.
TLDR
The sequence and configuration of the tripeptide, D-Phe-D-ALa-Trp, which was shown to have antibacterial activity was revealed and the relevance of the findings is discussed in respect to host-pathogen interactions. Expand
Champacyclin, a New Cyclic Octapeptide from Streptomyces Strain C42 Isolated from the Baltic Sea
TLDR
The compound (1a) showed antimicrobial activity against the phytopathogen Erwinia amylovora causing the fire blight disease of certain plants, and is likely synthesized by a non-ribosomal peptide synthetase (NRPS), because of its high content of (d)-amino acids. Expand
Curvopeptin: A New Lanthionine‐Containing Class III Lantibiotic and its Co‐substrate Promiscuous Synthetase
TLDR
The discovery and characterization of curvopeptins produced by Thermomonospora curvata, the first class III lantibiotics of thermophilic origin, are described and the surprising promiscuity of the Ser/Thr‐kinase domain is underlined. Expand
Deuterium labeled peptides give insights into the directionality of class III lantibiotic synthetase LabKC.
TLDR
A deuterium-label based enzyme assay is presented, utilizing a series of peptide substrates with α-deuterated Ser, for the determination of the dehydration order during the biosynthesis of class III lantibiotic labyrinthopeptin A2, and shows that, in contrast to other modifying enzymes of class I and II lantIBiotics, LabKC has a C- to N-terminal processing mode. Expand
Elucidation of sevadicin, a novel non-ribosomal peptide secondary metabolite produced by the honey bee pathogenic bacterium Paenibacillus larvae.
TLDR
The sequence and configuration of the tripeptide, D-Phe-D-ALa-Trp, which was shown to have antibacterial activity was revealed and the relevance of the findings is discussed in respect to host-pathogen interactions. Expand
Total synthesis of the ribosomally synthesized linear azole-containing peptide plantazolicin A from Bacillus amyloliquefaciens.
Total Synthesis of the Ribosomally Synthesized Linear Azole-Containing Peptide Plantazolicin A from Bacillus amyloliquefaciens A cyclodehydration reaction has been used to synthesize the linearExpand
Dissecting reactions of nonlinear precursor peptide processing of the class III lanthipeptide curvopeptin.
TLDR
These results point to a nonlinear processing scheme with a predominant C → N-terminal directionality of biosynthetic processing of the class III lanthipeptide labyrinthopeptin. Expand
...
1
2
...