Paul D. Hoeprich

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Insertion of membrane proteins into discoidal membranes using a cell-free protein expression approach.

Federico Katzen, Julia E. Fletcher, +9 authors Wieslaw A Kudlicki
Journal of proteome research
2008

We report a cell-free approach for expressing and inserting integral membrane proteins into water-soluble particles composed of discoidal apolipoprotein-lipid bilayers. Proteins are inserted into the… (More)

Cell-free co-expression of functional membrane proteins and apolipoprotein, forming soluble nanolipoprotein particles.

Jenny A. Cappuccio, Craig D. Blanchette, +14 authors Matthew A. Coleman
Molecular & cellular proteomics : MCP
2008

Here we demonstrate rapid production of solubilized and functional membrane protein by simultaneous cell-free expression of an apolipoprotein and a membrane protein in the presence of lipids, leading… (More)

Conjugation to nickel-chelating nanolipoprotein particles increases the potency and efficacy of subunit vaccines to prevent West Nile encephalitis.

Nicholas O. Fischer, Ernesto Infante, +4 authors Peter W. Mason
Bioconjugate chemistry
2010

Subunit antigens are attractive candidates for vaccine development, as they are safe, cost-effective, and rapidly produced. Nevertheless, subunit antigens often need to be adjuvanted and/or… (More)

Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.

Roy Lachica, C A Genigeorgis, Paul D. Hoeprich
Applied microbiology
1971

Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations… (More)

Obfuscation of the activity of antifungal antimicrobics by culture media.

Paul D. Hoeprich, Patricia D Finn
The Journal of infectious diseases
1972

Different apolipoproteins impact nanolipoprotein particle formation.

Brett Chromy, Erin S Arroyo, +13 authors Paul D. Hoeprich
Journal of the American Chemical Society
2007

Spontaneous interaction of purified apolipoproteins and phospholipids results in formation of lipoprotein particles with nanometer-sized dimensions; we refer to these assemblies as nanolipoprotein… (More)

Effect of culture media on the antifungal activity of miconazole and amphotericin B methyl ester.

Paul D. Hoeprich, Alan Huston
The Journal of infectious diseases
1976

The capacity of four culture media to obfuscate the antifungal activity of miconazole and amphotericin B methyl ester was evaluated qualitatively by examination of five isolates each of Candida… (More)

Hydrogen production by a hyperthermophilic membrane-bound hydrogenase in water-soluble nanolipoprotein particles.

Sarah E. Baker, Robert C. Hopkins, +10 authors Paul T Henderson
Journal of the American Chemical Society
2009

Hydrogenases constitute a promising class of enzymes for ex vivo hydrogen production. Implementation of such applications is currently hindered by oxygen sensitivity and, in the case of… (More)

Tolerance of staphylococcal thermonuclease to stress.

Roy Lachica, Paul D. Hoeprich, Helge Riemann
Applied microbiology
1972

Remarkable tolerance to prolonged heating, prolonged storage, and bacterial proliferation was exhibited by staphylococcal thermonuclease in foods and broth. A purified enzyme preparation added to… (More)

Alanine: Cycloserine Antagonism. Ii. Significance of Phenomenon to Therapy with Cycloserine.

Paul D. Hoeprich
Archives of internal medicine
1963

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