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Neural crest (NC) cells have been elegantly traced to follow stereotypical migratory pathways throughout the vertebrate embryo, yet we still lack complete information on individual cell migratory behaviors and how molecular mechanisms direct NC cell guidance. Here, we analyze the spatio-temporal migratory pattern of post-otic NC and the in vivo role of the(More)
A computer-controlled microscopy system was devised to allow the observation of avian embryo development over an extended time period. Parallel experiments, as well as extended specimen volumes, can be recorded at cellular resolution using a three-dimensional scanning procedure. The resulting large set of data is processed automatically into registered,(More)
Monitoring morphogenetic processes, at high resolution over time, has been a long-standing goal of many developmental cell biologists. It is critical to image cells in their natural environment whenever possible; however, imaging many warm-blooded vertebrates, especially mammals, is problematic. At early stages of development, birds are ideal for imaging,(More)
Extracellular matrix (ECM) movements and rearrangements were studied in avian embryos during early stages of development. We show that the ECM moves as a composite material, whereby distinct molecular components as well as spatially separated layers exhibit similar displacements. Using scanning wide field and confocal microscopy we show that the velocity(More)
INTRODUCTIONCell migration is a key aspect of many developmental processes, yet there are relatively few whole-vertebrate embryo culture systems that allow for intravital, high-resolution optical imaging of cell movements, cell-cell interactions, and cell-matrix interactions. Here, we present a protocol for 4D (3D + time), high-resolution confocal imaging(More)
The formation of a primary vascular bed is a dynamic process, aspects of which are readily amenable to time-lapse imaging in avian embryos. At early developmental stages, the body plan of avian embryos is very similar to mammals and has many properties that make it ideal for imaging. We devised labeling, culturing, and imaging techniques that capture(More)
Near-fields of non-resonantly laser-excited nanostructures enable strong localization of ultrashort light fields and have opened novel routes to fundamentally modify and control electronic strong-field processes. Harnessing spatiotemporally tunable near-fields for the steering of sub-cycle electron dynamics may enable ultrafast optoelectronic devices and(More)
gastrulation: combined roles for integrin α(5)β(1), fibronectin, and tissue geometry. The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development. time-lapse analysis of chick hindbrain neural crest cell migration shows cell interactions during migration to the branchial arches. Development 127:1161-1172. 11.Little,(More)
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