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A 7.5 kbp cryptic plasmid is found in almost all isolates of Chlamydia trachomatis. Real-time PCR assays, using TaqMan chemistry, were set up to quantify accurately both the chlamydial plasmid and the single copy, chromosomal omcB gene in the infectious, elementary bodies (EBs) of C. trachomatis L1 440. Plasmid copy number was also determined in the EBs of… (More)
BACKGROUND In early 2002 reports of outbreaks of gastroenteritis reached unprecedented levels in the UK. Forty five Norovirus outbreaks were reported in January 2002. OBJECTIVES The objective of the study was to determine whether the outbreaks were Noroviral in origin and if so whether they represented a homogeneous or heterogeneous collection of… (More)
Stools from 56 patients with gastroenteritis were cultured for Campylobacter jejuni. The five strains isolated were examined by electron microscopy. The campylobacter cells were pleomorphic and most displayed appearances similar to those of V. fetus. Morphological changes were observed in cultures subjected to prolonged incubation.
We examined some epidemiological features of the viruses associated with gastrointestinal illness, using national data reported by electron microscopists in the United Kingdom. During the 3 years analyzed (1985-1987), a total of 1,993 positive detections of astroviruses, caliciviruses, coronaviruses, and small round structured viruses (SRSVs) were reported.… (More)
Daily and weekly bag-changing regimens were compared in 12 elderly catheterised patients with reference to their clinical condition, leakage or blockage of the catheters, and the bacteriological state of the urine. Latex Foley catheters were used for the first 3 months and silastic catheters for the second 3 months of study. All patients had bacteriuria… (More)
An outbreak of gastroenteritis involving residents and members of staff in a nursing home for the elderly is described. The agent associated with this episode was a calicivirus which is serologically distinct from two strains causing gastroenteritis in children. We believe that this is the first report of calicivirus infection in adults.
A study was made of 20 patients fulfilling the criteria customarily used for the diagnosis of interstitial cystitis. A possible infective aetiology was sought by culture of bladder tissue, catheter and midstream specimens of urine, and urethral swabs by methods capable of detecting fastidious bacteria as well as aerobic pathogens. All bladder biopsies… (More)