Patrick Buehler

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The goal of this work is to detect hand and arm positions over continuous sign language video sequences of more than one hour in length. We cast the problem as inference in a generative model of the image. Under this model, limb detection is expensive due to the very large number of possible configurations each part can assume. We make the following(More)
The goal of this work is to automatically learn a large number of British sign language (BSL) signs from TV broadcasts. We achieve this by using the supervisory information available from subtitles broadcast simultaneously with the signing. This supervision is both weak and noisy: it is weak due to the correspondence problem since temporal distance between(More)
The goal of this work is to detect and track the articulated pose of a human in signing videos of more than one hour in length. In particular we wish to accurately localise hands and arms, despite fast motion and a cluttered and changing background. We cast the problem as inference in a generative model of the image, and propose a complete model which(More)
UNLABELLED Prostate-specific membrane antigen (PSMA), a transmembrane glycoprotein, is highly expressed by virtually all prostate cancers and is currently the focus of several diagnostic and therapeutic strategies. We have previously reported on the generation of several monoclonal antibodies (mAb) and antibody fragments that recognize and bind with high(More)
BACKGROUND For redirecting T-lymphocytes to induce prostate cancer cell lysis, we constructed a novel bispecific single-chain (bsc) diabody directed to the prostate specific membrane antigen (PSMA) and the T-cell receptor (TCR)-associated CD3 molecule on T-cells. METHODS The PSMA × CD3 bsc diabody was generated from an anti-CD3 single chain Fv fragment(More)
Prostate cancer is the most common cancer in men from Western industrialized countries and a significant proportion of patients progress to advanced metastatic disease, for which currently no curative treatment exists. Therefore, new therapeutic approaches need to be considered. Prostate specific membrane antigen (PSMA) is an integral, non-shed type 2(More)
AIM Although prostate cancer is one of the most commonly diagnosed malignancies in men, there is no effective curative therapy for the advanced disease. Therefore, the aim of the present study was to generate prostate-specific membrane antigen (PSMA)×CD3 diabodies as a novel treatment option for this tumor. METHODS A PSMA×CD3 diabody and a covalently(More)
BACKGROUND Prostate-specific membrane antigen (PSMA) is an excellent candidate for targeting prostate cancer by virtue of its restricted expression on prostatic epithelial cells and its upregulation on prostatic carcinoma cells. PSMA is expressed on the cell surface displaying a specific three-dimensional structure. Therefore, only antibodies with a high(More)
BACKGROUND Radioimmunoimaging with disease-specific tracers can be advantageous compared to that with nonspecific tracers for the imaging of glucose metabolism and cell proliferation. Monoclonal antibodies (mAbs) or their fragments are excellent tools for immuno-positron emission tomography (PET). In this study, PSMA-specific mAb 3/F11 and its recombinant(More)
Recently, we have described a bispecific PSMA x CD3 diabody with one binding site for the T-cell antigen receptor (TCR-CD3) and another for the Prostate Specific Membrane Antigen (PSMA). It effectively eliminates human prostate cancer cells by redirecting T-lymphocytes in vitro and in vivo. Here, we show that activation of the T-cells and killing of the(More)