Patricia Mire

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Sea anemones are sessile invertebrates that detect movements of prey using numerous hair bundles located on tentacles surrounding their mouth. Previously we found that hair bundles of anemones are structurally and functionally similar to those of vertebrates. After 10-15 min exposure to calcium depleted buffers, hair bundles in chickens suffer moderate(More)
Sea anemones are marine invertebrates that use hair bundles to detect swimming movements of prey. Prey are captured by nematocysts (stinging capsules) that discharge into the prey. To further characterize anemone hair bundles and to compare hair bundles in anemones with hair bundles in vertebrates, we investigated fine structure and cytochemistry of anemone(More)
Hair bundles on tentacles of sea anemones are similar to vertebrate hair bundles in terms of structure and function. Anemone hair bundles are involved in regulating discharge of nematocysts, "stinging capsules," used to capture prey. N-acetylated sugars from the prey including N-acetylneuraminic acid (NANA) induce hair bundles to elongate while shifting(More)
Although gap junctions occur in auditory and vestibular systems, their function is unclear. Here we present evidence for gap junctional communication in transmitting mechanosensory signals in a sea anemone model system. Hair bundles on anemone tentacles are vibration-sensitive mechanoreceptors that regulate discharge of nematocyst from effector cells. We(More)
Hair bundle mechanoreceptors can be damaged by over-stimulation or by exposure to calcium-free buffers. Provided the trauma is slight, hair bundles recover, although the subcellular mechanisms for such recovery are poorly understood. Hair bundle mechanoreceptors on tentacles of sea anemones are especially resilient, recovering from severe trauma within(More)
Most hair bundles are essentially fixed with respect to frequency specificity. However, hair bundles in sea anemones are dynamically tuned by actin-dependent changes in length. Tuning to low frequencies is accomplished by activation of chemoreceptors to N-acetylated sugars resulting in hair bundle elongation. We report here that following sugar-induced(More)
Hair bundle mechanoreceptors of the acousticolateralis system of vertebrates are similar to hair bundles found on tentacles of sea anemones, primitive marine invertebrates. In each case, hair bundles consist of actin-based stereocilia interconnected by extracellular linkages. Recently, considerable attention has been directed to one class of linkages called(More)
Sea anemones are among the simplest animals to use hair bundles to detect vibrations. Although we previously found anemone bundles to be morphologically similar to vertebrate hair bundles, only indirect evidence implicated anemone bundles in mechanotransduction. Here, we test mechanotransduction of these bundles using loose-patch current recording from(More)
A homolog of TRPA1 was identified in the genome of the anemone, Nematostella vectensis (nv-TRPA1a), and predicted to possess six ankyrin repeat domains at the N-terminus and an ion channel domain near the C-terminus. Transmembrane segments of the ion channel domain are well conserved among several known TRPA1 polypeptides. Inhibitors of TRPA1 including(More)
The sea anemone Haliplanella luciae (Cnidaria, Anthozoa) detects chemical and mechanical stimuli from prey. Hair bundle mechanoreceptors on the tentacles participate in regulating discharge of microbasic p-mastigophore nematocysts. Properly stimulated hair bundles sensitize the anemone to discharge nematocysts into objects that contact the tentacles. The(More)