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BACKGROUND In chronic wounds, biofilms probably play a vital role in protecting bacteria from host defenses and antimicrobial medications by creating a barrier of exopolysaccharide that is difficult for the immune system and antibiotics to penetrate. A biofilm consists of an exopolysaccharide matrix that is produced and secreted by certain species of(More)
Culture of the human neoplastic submandibular gland intercalated duct cell line, HSG, on the basement membrane extract Matrigel induces dramatic morphologic changes and cytodifferentiation. Transmission electron microscopy demonstrated an acinar cell phenotype with polarized cells containing a well-developed Golgi apparatus, multiple microvilli-like(More)
A biofilm is a collection of microbial cells that are attached to a surface and embedded in a self-produced extrapolymeric substance. The understanding of the biofilm phenotype is important in the understanding of bacteria in vitro but it has been difficult to translate biofilm science to the clinical setting. More recently, preliminary criteria for(More)
The medical literature describes numerous in vitro and in vivo wound-healing models. The selection of an animal model depends on a number of factors including availability, cost, ease of handling, investigator familiarity, and anatomical/functional similarity to humans. Small mammals are frequently used for wound healing studies, however, these mammals(More)
We studied the effects of direct electric current supplied by an energized silver-coated electrode on dermal and epidermal wound healing. Keratome-induced wounds (0.3 mm deep) on the skin of young domestic pigs were treated with either an energized (50-300 microA) electrode (DC), an unenergized electrode (placebo), or left untreated. Wounds were excised on(More)
We examined the effect of delayed application and early removal of a polyurethane dressing on excisional wounds in swine. Backs of pigs were wounded with an electrokeratome, and wounds were divided into the following treatment groups: (1) air exposed; (2) dressings applied immediately after wounding and kept on until wounds were evaluated; (3) dressings(More)
The effects on superficial wounds in domestic pigs of (1) two different occlusive dressings, (2) non-occlusive wet to dry gauze dressings, and (3) air exposure were compared. Collagen synthesis and re-epithelialization were increased in the wounds treated with occlusive dressings. Re-epithelialization was increased beneath both the oxygen-impermeable and(More)
Previous in vitro studies suggest that the interleukin-1 (IL-1) proteins may be important for skin function. Keratinocytes are known to synthesize IL-1 in response to injury and IL-1 has been shown to stimulate fibroblast and keratinocyte growth, collagen synthesis by fibroblasts, and chemotaxis of keratinocytes. In order to evaluate the ability of IL-1 to(More)
Monocytes/macrophages are associated with chronic inflammatory lesions, such as periodontal disease and rheumatoid arthritis, in which there is extensive connective tissue destruction. Stimulation of human monocytes results in the production of matrix metalloproteinases (MMPs) via a prostaglandin E2 (PGE2)-cAMP-dependent pathway. Modulation of many monocyte(More)
Because occlusive dressings and some creams have been found to speed epithelialization of blade-induced wounds, we studied the effect of two occlusive dressings and a polyglycerylmethacrylate cream containing low concentration of fibronectin on epithelialization in second-degree burn wounds. Cylindrical brass rods were heated in a boiling water bath,(More)