Patricia Hare

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O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.
Hydrolyzates of aqueous extracts of Apollo 11 fines, an Apollo 12 trench sample, and an Apollo 12 surface sample have been analyzed on an ultrasensitive amino acid analyzer. The total content of amino acids recovered ranged from 20 to 70 parts per billion of lunar soil. Amino acids are not recovered by the direct hydrolysis of lunar fines, presumably(More)
Hydroxylysine and hydroxyproline are absent in the calcified proteins of Mytilus. The organic matrices from the calcite layers have a consistently higher ratio of acidic to basic amino acids than the aragonitic shell units. The uncalcified shell units periostracum and outer ligament, have very few acidic residues, which may in part account for the lack of(More)
Eggshells of the African ostrich (Struthio camelus), ubiquitous in archeological sites in Africa, have been shown by laboratory simulation experiments to retain their indigenous organic matrix residues during diagenesis far better than any other calcified tissue yet studied. The rate of L-isoleucine epimerization to D-alloisoleucine follows reversible(More)
The production of an extracellular collagenase and an alkaline protease by Vibrio alginolyticus during stationary phase was inhibited by a temperature shift from 30 to 37°C and by a lack of oxygen. The stability of the exoproteases was unaffected by incubation at 37°C and aeration. The optimum growth temperature for the V. alginolyticus strain was 33.5°C(More)
Laboratory models of the origin of living systems have demonstrated that proteinlike polymers easily arise in substantial yield by the simple heating of e-amino acids (Fox, 1964). Once formed, such polymers should be stable in the dry crust of the Moon. Appropriate methods of extraction and hydrolysis of such polymers should then yield amino acids. When(More)