Patricia A. Schachern

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To our knowledge, the normal changes that occur in the tympanic membrane after birth have not been described, despite several reports that their otoscopic appearance in healthy, full-term infants is different from that in adults. To provide such a description, the tympanic membranes of 54 temporal bones that had no pathologic evidence of otologic diseases,(More)
The Sleeping Beauty (SB) transposon system is a nonviral vector that directs transgene integration into vertebrate genomes. We hydrodynamically delivered SB transposon plasmids encoding human alpha-L-iduronidase (hIDUA) at two DNA doses, with and without an SB transposase gene, to NOD.129(B6)-Prkdc(scid) IDUA(tm1Clk)/J mice. In transposon-treated, nonobese(More)
BACKGROUND The Sleeping Beauty (SB) transposon system is a non-viral vector system that can integrate precise sequences into chromosomes. We evaluated the SB transposon system as a tool for gene therapy of mucopolysaccharidosis (MPS) types I and VII. METHODS We constructed SB transposon plasmids for high-level expression of human beta-glucuronidase(More)
OBJECTIVES To compare histopathological and clinical findings of metastasis to the temporal bone with previous reports and to determine the prevalence of these metastases in patients with nonsystemic cancer. STUDY DESIGN Retrospective. METHODS Autopsy records of 864 patients were screened to select those with primary nondisseminated malignant neoplasms.(More)
OBJECTIVES Previous reports on aging of human cochlea included subjects with ear diseases or ototoxic drugs. We studied spiral ganglion cells, hair cells, and lateral wall of cochlea from subjects without ear disease or ototoxic drugs. STUDY DESIGN This study included 39 temporal bones from 24 subjects aged 1 day to 86 years. We assessed standard(More)
Fabry's disease is a rare progressive X-linked recessive disorder of glycosphingolipid metabolism. The accumulation of glycosphingolipids occurs in virtually all areas of the body, including the endothelial, perithelial, and smooth-muscle cells of blood vessels, the ganglion cells of the autonomic nervous system, and the glomeruli and tubules of the kidney.(More)
PURPOSE A high jugular bulb (JB) is thought to affect structures of the inner ear and possibly cause symptoms there, but clear histological findings of an anatomical relationship between a high JB and the inner ear have not yet been described. MATERIALS AND METHODS We surveyed horizontal sections of 1,591 temporal bones from the collection of the(More)
OBJECTIVE To study temporal bone histopathologic characteristics of the inner ear and middle ear cleft of patients with Down's syndrome. DESIGN Sixteen temporal bones from eight patients with Down's syndrome were studied. Ten temporal bones from subjects without pathologic ear lesions but with congenital heart disease served as controls. The(More)
OBJECTIVES To identify the mucin gene and its expressing cells in the middle ear mucosa with chronic otitis media (COM), and to study the correlation between infiltration of inflammatory cells in the submucosa and expression of the mucin gene in the mucosal epithelium with COM. STUDY DESIGN Middle ear mucosal specimens removed from the inferior promontory(More)
The thickness and morphologic characteristics of the round window membrane were evaluated in temporal bones from normal subjects as well as those with serous otitis media, purulent otitis media, and chronic otitis media. Temporal bones were studied in chronological order in six age ranges to determine the possibility of age-related differences. No(More)