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The levels of magnesium (Mg), zinc (Zn) and copper (Cu) in the plasma, erythrocytes (RBC) and polymorphonuclear (PMN) and lymphocyte (L), leukocytes (WBC) of 45 diabetic children were compared to those of 12 normal children and were related to the diabetic control via HbA1 and fasting blood sugar (FBS) assessments. Mineral levels were determined via(More)
We determined selenium concentrations and activities of the selenoenzyme, glutathione peroxidase (EC, in the plasma and erythrocytes of 38 apparently healthy women. We determined selenium concentrations directly by polarized Zeeman-effect flameless atomic absorption spectroscopy. Within-run precision studies for the assays gave CVs of 5.6% for a(More)
The effects of trace metal nutrition on the fetus and neonate have been described. Since very little research has been done with the human fetus and neonate, much of our knowledge must be extrapolated from animal studies. In addition, most of the work centres around the effects of copper and zinc nutrition. Nutritional requirements (when known) for both(More)
We describe methods for determination of manganese in whole blood and serum with Zeeman-effect flameless atomic absorption spectroscopy. These analyses are performed on a twofold or fourfold dilution of the specimen in Triton X-100, 1 g/L. No predigestion or extraction procedures are required. The method of standard additions was used for quantitation.(More)
Direct determination of cadmium in whole blood and urine can be achieved using Zeeman effect flameless atomic absorption spectroscopy. Within-run precision studies for whole blood cadmium determinations gave relative standard deviations of 11.3% and 6.3% for 0.53 micrograms/l and 3.16 micrograms/l, respectively. Within-run precision studies for the urine(More)
To determine whether a population living on or adjacent to four sites of chromate production waste was measurably exposed to environmental chromium, spot samples of urine were collected along with wipe samples of household dust and lifestyle/activity interview data. Findings were compared to those from a control population in two communities with no(More)
Methodologies for the analysis of cadmium, copper, iron, lead, selenium and zinc in human seminal plasma and spermatozoa have been developed. Analyses were made directly in a dilution of seminal plasma or nitric acid digest of lyophilized cells using Zeeman-effect atomic absorption spectroscopy. Within-run coefficients of variation (CV's) for pooled(More)
Nonsteroidal biochemical markers of oocyte maturity could presumably aid in oocyte selection and in the timing of insemination for in vitro fertilization. We assessed the usefulness of six potential markers found in follicular fluid. We used kinetic immunonephelometry to measure concentrations of alpha 1-antitrypsin, alpha 2-macroglobulin, antithrombin III,(More)
The oxidation products of selenomethionine (SeMet) have been studied via experimental (77)Se NMR and theoretical (77)Se chemical shifts. Four signals are observed: a diastereomeric pair of selenoxides at 840 ppm and two unidentified resonances at 703 and 716 ppm. Theoretical DeltaG and chemical shifts suggest the 703 and 716 ppm resonances correspond to(More)