Patricia A. Katomski

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Loss of tritium from specific positions in [3H,14C] aromatic hydrocarbons can elucidate their binding site(s) to DNA and RNA and indicate the mechanism of activation. Studies of tritium loss from [6-3H,14C]benzo[a]pyrene (B[a]P), [1,3-3H,14C]B[a]P, [1,3,6-3H,14C]B[a]P, [6,7-3H,14C]B[a]P, and [7-3H,14C]B[a]P were conducted in vitro using liver nuclei and(More)
Covalent binding of aromatic hydrocarbons to DNA is catalyzed by horseradish peroxidase with HzOz. Under optimal reaction conditions (30 PM hydrocarbon, 5 x lo-“ M HzOz, and 0.2 mg/ml of horseradish peroxidase at pH 7.0), binding was linear for 30 min. Binding of benzo[a]pyrene, 6-methylbenzo[a]pyrene, and 3-methylcholanthrene ranged from 40 to 100 pmol/mol(More)
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