Patrícia Alexandra Pereira

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Arbuscular mycorrhizae (AM) are the most widespread symbioses on Earth, promoting nutrient supply of most terrestrial plant species. To unravel gene expression in defined stages of Medicago truncatula root colonization by AM fungi, we here combined genome-wide transcriptome profiling based on whole mycorrhizal roots with real-time reverse transcription-PCR(More)
Most of the core proteins involved in the microRNA (miRNA) pathway in plants have been identified, and almost simultaneously hundreds of miRNA sequences processed in the Arabidopsis sporophyte have been discovered by exploiting next-generation sequencing technologies. However, there is very limited understanding about potentially distinct mechanisms of(More)
Legumes have the unique ability to host nitrogen-fixing Rhizobium bacteria as symbiosomes inside root nodule cells. To get insight into this key process, which forms the heart of the endosymbiosis, we isolated specific cells/tissues at different stages of symbiosome formation from nodules of the model legume Medicago truncatula using laser-capture(More)
OBJECTIVE To characterize circulating B-cell subpopulations of arthritis patients with <6 weeks of disease duration. METHODS Peripheral blood samples were collected from very early untreated polyarthritis patients, with <6 weeks of disease duration, for flow cytometric evaluation of B-cell subpopulations. Samples from patients who were later diagnosed as(More)
Glutamine synthetase (GS) is a vital enzyme for the assimilation of ammonia into amino acids in higher plants. In legumes, GS plays a crucial role in the assimilation of the ammonium released by nitrogen-fixing bacteria in root nodules, constituting an important metabolic knob controlling the nitrogen (N) assimilatory pathways. To identify new regulators of(More)
BACKGROUND Cervical range of motion (CROM) is a fundamental component of the functional evaluation in physical therapy interventions. The CROM device stands out as a reliable, non-invasive and easy-to-use method, but it is a very expensive tool. In clinical practice, more affordable tools such as Fleximeters are preferred. However, the reliability of(More)
Background: Cervical range of motion (CROM) is a fundamental component of the functional evaluation in physical therapy interventions. The CROM device stands out as a reliable, non-invasive and easy-to-use method, but it is a very expensive tool. In clinical practice, more affordable tools such as Fleximeters are preferred. However, the reliability of(More)