Partha Pratim Mondal

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We propose a maximum a posteriori image restoration approach to 3D confocal microscopy. The image field is suitably modeled as a Markov random field, resulting in a Gibbs distributed image. A fuzzy-logic-based potential is employed in the Gibbs prior. Unlike other potentials, the fuzzy potential distinguishes intensity variation due to genuine edges and(More)
Positron emission tomography (PET) is one of the key molecular imaging modalities in medicine and biology. Penalized iterative image reconstruction algorithms frequently used in PET are based on maximum-likelihood (ML) and maximum a posterior (MAP) estimation techniques. The ML algorithm produces noisy artifacts whereas the MAP algorithm eliminates noisy(More)
Fast three-(3D) imaging requires parallel optical slicing of a specimen with an efficient detection scheme. The generation of multiple localized dot-like excitation structures solves the problem of simultaneous slicing multiple specimen layers, but an efficient detection scheme is necessary. Confocal theta detection (detection at 90° to the optical axis)(More)
We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system and is capable of 3D volume imaging by simultaneously illuminating multiple planes in the sample. Theta(More)
Monitoring and visualizing specimens at a large penetration depth is a challenge. At depths of hundreds of microns, several physical effects (such as, scattering, PSF distortion and noise) deteriorate the image quality and prohibit a detailed study of key biological phenomena. In this study, we use a Bessel-like beam in-conjugation with an orthogonal(More)
12 A technique for resolution improvement in two-photon excitation (2PE) fluorescence microscopy based on radially-symmetric annu-13 lar binary filter (consist of central circular aperture and a concentric peripheral annulus) is proposed. Resolution improvement is 14 achieved by engineering the aperture of the objective lens in a way so as to enhance high(More)
The authors show that the conditional entropy maximisation algorithm is a generalised version of the maximum likelihood algorithm for positron emission tomography (PET). Promising properties of the conditional entropy maximisation algorithm are as follows: an assumption is made that the entropy of the information content of the data should be maximised; it(More)
Temporal resolution is a key factor for imaging rapidly occurring events in biology. In this feature article, I investigate an approximate estimate for determining the temporal resolution limit. The condition that led to this limit is, the time taken by the ensemble (99.9%) of excited molecules to relax to ground state, assuming all the emitted photons are(More)
Appropriate PSF engineering in microscopy can be used to increase resolution [1], reduce aberrations [2] or to extend the depth-of-field (DOF) [3,4,5]. This study concentrates on PSF engineering in EDF microscopy. Most extended DOF techniques require image restoration by e.g. regularized filtering to achieve high lateral resolution and extended DOF(More)
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