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We propose a maximum a posteriori image restoration approach to 3D confocal microscopy. The image field is suitably modeled as a Markov random field, resulting in a Gibbs distributed image. A fuzzy-logic-based potential is employed in the Gibbs prior. Unlike other potentials, the fuzzy potential distinguishes intensity variation due to genuine edges and(More)
Fast three-(3D) imaging requires parallel optical slicing of a specimen with an efficient detection scheme. The generation of multiple localized dot-like excitation structures solves the problem of simultaneous slicing multiple specimen layers, but an efficient detection scheme is necessary. Confocal theta detection (detection at 90° to the optical axis)(More)
We developed a multiple light-sheet microscopy (MLSM) system capable of 3D fluorescence imaging. Employing spatial filter in the excitation arm of a SPIM system, we successfully generated multiple light-sheets. This improves upon the existing SPIM system and is capable of 3D volume imaging by simultaneously illuminating multiple planes in the sample. Theta(More)
The expectation maximization (EM) algorithm is extensively used for tomographic image reconstruction based on Positron Emission Tomography (PET) modality. The EM algorithm gives good reconstructed images compared to those created by deterministic methods such as Filtered Back Projection (FBP) and Convolution Back projection (CBP). However, the computational(More)
Monitoring and visualizing specimens at a large penetration depth is a challenge. At depths of hundreds of microns, several physical effects (such as, scattering, PSF distortion and noise) deteriorate the image quality and prohibit a detailed study of key biological phenomena. In this study, we use a Bessel-like beam in-conjugation with an orthogonal(More)
12 A technique for resolution improvement in two-photon excitation (2PE) fluorescence microscopy based on radially-symmetric annu-13 lar binary filter (consist of central circular aperture and a concentric peripheral annulus) is proposed. Resolution improvement is 14 achieved by engineering the aperture of the objective lens in a way so as to enhance high(More)
OBJECTIVES To assess the long term complications of JJ stent, the management of complications and the role of endoscopic approach to manage these complications. MATERIALS AND METHODS Nineteen patients with indwelling JJ stent for a duration of more than 6 months were included in this study. Patients were assessed with X-ray KUB, USG KUB, blood urea,(More)
This paper presents a simple unknown input estimation technique using generalized matrix inverse. The full order observer constructed by g-inverse is extended and implemented for this purpose. The necessary and sufficient condition has been introduced for existence of such an estimator. The proposed method is illustrated by numerical example (Two loop(More)
Appropriate PSF engineering in microscopy can be used to increase resolution [1], reduce aberrations [2] or to extend the depth-of-field (DOF) [3,4,5]. This study concentrates on PSF engineering in EDF microscopy. Most extended DOF techniques require image restoration by e.g. regularized filtering to achieve high lateral resolution and extended DOF(More)