Paricherer Ahmadian

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Pollen embryogenesis has been induced inCapsicum annuum L., selecting the late vacuolate microspore as the suitable developmental stage for the induction. Various modern in situ methods including cytochemistry, immunocytochemistry, and in situ hybridization have been used to characterize the functional organization of the microspore nucleus at this specific(More)
The immunolocalization of nuclear antigens, combined with cytochemical procedures as well as in situ hybridization and recent in situ molecular methods, has been applied at different steps of pollen development to characterize the functional organization of the nucleus during the formation of the male gametophyte in an agronomically interesting plant,(More)
Methylation-acetylation (MA) is an easy and reproducible ultrastructural cytochemical method which gives preferential contrast to nucleic acid containing structures. When performed en bloc before Lowicryl embedding it does not affect the main antigenic and chemical properties of the sample and is compatible with a large variety of modern immunogold methods(More)
The induction of pollen embryogenesis in Capsicum annuum L. has been studied at the cellular level using various in situ approaches with several molecular probes for DNA, RNA and proteins. The late vacuolated microspore and the young bicellular pollen grain are stages of gametophytic development in which embryogenesis can be induced. Our results show that(More)
Here we report for the first time the ultrastructural localization of DNA replication sites in the nucleus of plant cells and the timing of replication through the pollen developmental programme by proliferating cell nuclear antigen (PCNA) immunogold labelling. Replication sites were identified by labelling with anti-PCNA antibodies in fibrils of the(More)
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