Parameswaran Ramachandran

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The so-called receiver operating characteristic technique is used as a tool in an optimization procedure for the improvement and assessment of a filter-based methodology for the location of hot spots in protein sequences and exons in DNA sequences. By optimizing the characteristic values of the nucleotides, high efficiency as well as improved accuracy can(More)
A technique for the identification of hot-spot locations in proteins using digital filters is described. In this technique, the characteristic frequency of the protein sequence of interest is first determined from the consensus spectrum of the corresponding functional group. The sequence is then filtered by using a specialized narrowband bandpass digital(More)
MOTIVATION Reliable estimation of the mean fragment length for next-generation short-read sequencing data is an important step in next-generation sequencing analysis pipelines, most notably because of its impact on the accuracy of the enriched regions identified by peak-calling algorithms. Although many peak-calling algorithms include a fragment-length(More)
A filtering technique for the location of hot spots in proteins proposed recently is applied for the location of exons in DNA sequences. The technique involves conversion of a DNA character sequence into a numerical sequence using the electron-ion interaction potential values and then filtering the numerical sequence using a narrowband bandpass digital(More)
Microtubules are involved in numerous cellular processes including chromosome segregation during mitosis and, as a result, their constituent protein, tubulin, has become a successful target of several chemotherapeutic drugs. In general, these drugs bind indiscriminately to tubulin within both cancerous and healthy cells, resulting in unwanted side effects.(More)
An improved technique for the location of hot spots in proteins based on the use of a bandpass notch (BPN) filter is described. The BPN filter is designed by specifying a stability margin and then minimizing the area under the amplitude response so as to achieve maximum selectivity for the chosen stability margin. Preliminary results indicate that the use(More)
Unraveling transcriptional regulatory networks is a central problem in molecular biology and, in this quest, chromatin immunoprecipitation and sequencing (ChIP-seq) technology has given us the unprecedented ability to identify sites of protein-DNA binding and histone modification genome wide. However, multiple systemic and procedural biases hinder(More)
An optimized numerical mapping scheme for achieving improved location of exons in DNA sequences using digital filters is proposed. Characteristic numerical values for the four nucleotides, referred to as pseudo-EIIP values, are obtained using a training procedure where the location accuracy is maximized using a quasi-Newton algorithm based on the(More)
Wnt signaling, named after the secreted proteins that bind to cell surface receptors to activate the pathway, plays critical roles both in embryonic development and the maintenance of homeostasis in many adult tissues. Two particularly important cellular programs orchestrated by Wnt signaling are proliferation and stem cell self-renewal. Constitutive(More)