Paolo Gargiulo

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A reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reductase with the ability to reduce diacetyl has been isolated from Escherichia coli and has been purified 800-fold to near homogeneity. The product of the reduction of diacetyl was shown to be acetoin. The enzyme proved to catalyze the oxidation of NADPH in the presence of both(More)
This study demonstrates in a novel way how volume and shape are restored to denervated degenerated muscles due to a special pattern of electrical stimulation. To this purpose, Spiral Computer Tomography (CT) and special image processing tools were used to develop a method to isolate the rectus femoris from other muscle bellies in the thigh and monitor(More)
This work develops a new method to study and analyze structural muscle changes resulting form electrical stimulation treatment. The muscular changes in denervated degenerate muscle undergoing Therapeutic Electrical Stimulation (TES) treatment are seen and quantified using a novel approach based on image processing tools and medical images. Spiral CT images(More)
Muscle tissue composition can be efficiently analyzed and quantified using images from spiral computed tomography technology (SCT) and the associated values of Hounsfield unit (HU) for different tissues. This work propose a novel approaches to monitor muscle condition in denervated muscle undergoing electrical stimulation (ES) treatment based on image(More)
Total hip arthroplasty (THA) can be achieved by using a cemented or noncemented prosthesis. Besides patient's age, weight, and other clinical signs, the evaluation of the quality of the bones is a crucial parameter on which orthopedic surgeons base the choice between cemented and noncemented THA. Although bone density generally decreases with age and a(More)
Total hip arthroplasty (THA) is performed with or without the use of bone cement. Facing the lack of reliable clinical guidelines on decision making whether a patient should receive THA with or without bone cement, a joint clinical and engineering approach is proposed here with the objective to assess patient recovery developing monitoring techniques based(More)
Despite the ravages of long term denervation there is structural and ultrastructural evidence for survival of muscle fibers in mammals, with some fibers surviving at least ten months in rodents and 3-6 years in humans. Further, in rodents there is evidence that muscle fibers may regenerate even after repeated damage in the absence of the nerve, and that(More)
In the frame of the EU-funded RISE project, patients with lower motor neuron lesion and denervated and degenerated muscles are treated with electrical stimulation, with the aim of restoring muscle mass and force. Spiral computer tomography from the hip joint down to the knee joint is used to gather three-dimensional data on the upper leg tissue. These data(More)
A novel clinical rehabilitation method for patients who have permanent and non recoverable muscle denervation in the legs was developed in the frame of the European Project RISE. The technique is based on FES and the project results shows, in these severely disabled patients, restoration of muscle tissue and function. This study propose novel methods based(More)
This study demonstrates qualitatively and quantitatively how functional electrical stimulation (FES) restores volume and morphology to denervated degenerate muscle. To this purpose a novel technique was developed to isolate and monitor muscle growth. During 3 years of electrical stimulation, complete 3-dimensional reconstructions of rectus femoris muscles(More)