Paolo Catalani

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Bone marrow stromal microenvironment is essential for the maintenance of the hematopoietic stem cell renewal both by cell-cell interaction and cytokine production. However, stromal cells also exhibit drug metabolizing activities and they may accumulate the drug and successively affect hematopoietic progenitors by a retarded release. Our study investigated(More)
The aim of this study was to evaluate the influence of the two-step hepatocyte isolation procedure on primary cultured trout (Oncorhynchus mykiss) hepatocytes over time. We characterised the possible changes of a variety of some cellular parameters within the first 24-48 h after seeding. We followed the time dependent changes of these parameters during(More)
In order to develop a method for detecting metabolism-mediated embryotoxicity, differentiating embryonic stem (ES) cells were exposed to the well-known proteratogen, cyclophosphamide (CPA). CPA was tested in a scientifically validated embryonic stem-cell test (EST), and in the newly developed reporter-gene assay for developmental cardiotoxicity. Both assays(More)
The covalent binding of hexamethylmelamine (HMM) and its metabolites was studied in liver, tumor, blood, kidney, spleen, lung, brain, heart, and small intestine after a single IP injection of 2,4,6-14C-hexamethylmelamine (50 mg/kg) to C57Bl/6J female mice bearing 20-day-old M5076/73A ovarian cancer. Covalent binding to tissue macromolecules was measured 2,(More)
A high-performance liquid chromatographic method has been developed for the simultaneous determination of alpidem and its metabolites in human plasma. The method involved a single extraction of the parent drug and metabolites into diethyl ether from alkalinized plasma, evaporation of the organic solution and chromatography of the extracts on a C18 column(More)
The metabolism of doxorubicin was studied in murine long-term bone marrow cultures (LTBMC) and in SR-4987 established stromal cells in comparison with primary cultures of murine and rat hepatocytes. The toxicity of metabolites was verified by testing their effects on the clonogenicity of granulo-macrophage progenitors. Metabolic activity was compared in(More)
Imipramine is metabolized in vitro by rat, mouse or human microsomes to several metabolites including 2-hydroxyimipramine, 2-hydroxydesipramine, desipramine, didesmethylimipramine and imipramine-N-oxide. Heat treatment (which inhibits FMO), verified the role of this group of enzymes in the oxidation of imipramine to imipramine N-oxide. These studies(More)
The effects of two substituted polychlorinated biphenyls, the 3,4,5,3',4,5' (PCB-169) and the 2,3,4,2',4',5' (PCB-138) forms, were examined on the expression of c-myc, c-jun, c-ras, and jun-b in 3T3-L1 cells. Northern blot analysis demonstrated that the two PCBs, which exhibit a coplanar and di-ortho-substituted configuration, activated these oncogenes(More)
A high-performance liquid chromatographic method has been developed for the determination of progabide and its main acid metabolite in blood, serum and plasma. The assay involved a single and rapid extraction of drug and metabolite into toluene from the biological matrix buffered at pH 4.8, evaporation of the organic phase, and chromatography of the(More)