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BACKGROUND The aim of the present study is to investigate cryopreservation of oocytes in patients refusing embryo cryopreservation for ethical reasons, patients from whom no sperm could be retrieved and patients with enough oocytes to yield a number of fresh and cryopreserved embryos to transfer. METHODS A total of 2900 oocytes out of 6216 retrieved were(More)
Cleavage kinetics of human embryos is indicative of ability to develop to blastocyst and implant. Recent advances in time-lapse microscopy have opened new and important research opportunities. In this study involving infertile couples requiring standard IVF/intracytoplasmic sperm injection treatment, zygotes were cultured by integrated embryo-culture(More)
OBJECTIVE To report the first birth after transfer of cryopreserved embryos generated by intracytoplasmic sperm injection of cryopreserved testicular spermatozoa into cryopreserved human oocytes. DESIGN Case report. SETTING Tertiary center for reproductive technology. PATIENT(S) A 36-year-old woman with primary infertility of 3 years' duration and a(More)
OBJECTIVES Conventional sperm freezing procedures need the addition of a relatively large volume of cryoprotectant. The dilution of extremely poor sperm suspensions from ejaculate or testicular tissue may make the recovery of viable spermatozoa difficult at the moment of the intracytoplasmic sperm injection (ICSI) procedure. The cryopreservation of a few(More)
BACKGROUND In May 2009, the Italian Constitutional Court banned most of the limitations of a restrictive law regulating assisted reproduction technology on the grounds that it limited a couple's right to have access to the best possible medical treatment and reduce any possible higher risk of complications. The aim of the study was to compare our results in(More)
BACKGROUND In a growth phase occurring during most of folliculogenesis, the oocyte produces and accumulates molecules and organelles that are fundamental for the development of the preimplantation embryo. At ovulation, growth is followed by a phase of maturation that, although confined within a short temporal window, encompasses modifications of the oocyte(More)
PURPOSE The aim of this retrospective study was to compare the competence of oocytes obtained from preovulatory and antral follicles. METHODS Mature oocytes from preovulatory follicles were retrieved from women selected for standard IVF treatment (Group A). Mature oocytes from antral follicles were recovered from women undergoing hCG-primed in vitro(More)
OBJECTIVE To investigate the consequences of a law introduced in Italy in 2004 that forbids the fertilization or injection of more than three oocytes for assisted reproduction and does not allow any embryo selection or cryopreservation. DESIGN Retrospective observational analysis. SETTING Subfertile patients enrolled in an assisted reproduction program.(More)
OBJECTIVE To evaluate the efficacy of a new ultravitrification technique with a low concentration of cryoprotectants. DESIGN Ultravitrification research. SETTING Private assisted reproduction center. PATIENT(S) Oocytes donated voluntarily with the aim of research. INTERVENTION(S) Ultravitrification with different protocols of 100 mature oocytes and(More)
BACKGROUND This study was carried out to evaluate whether bilateral orchidopexy represents a poor or good prognostic factor in azoospermic men undergoing testicular sperm extraction (TESE). METHODS One hundred and seven presumed non-obstructive azoospermia (NOA) patients, according to conventional clinical parameters (volume of testis, FSH, clinical(More)