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Monoclonal antibody (mAb) 5B4 recognizes a developmentally regulated membrane glycoprotein (Mr approximately 185,000-255,000) expressed on sprouting neurons. The amino acid sequence deduced from lambda gt11 cDNA clones encoding the transmembrane and cytoplasmic domains of the 5B4 antigen is co-linear with that of chick NCAM-ld. The significant level of(More)
A mutant human insulin receptor with a carboxyl-terminal deletion of 69 amino acids (proreceptor residues 1287-1355) is expressed as a stable protein in transiently transfected COS cells. We find that in intact cells this mutant is phosphorylated in an insulin-dependent manner on core tyrosines 1158, 1163 and 1163. As expected, the carboxyl-terminal(More)
Tetratricopeptide repeat domain 9A (TTC9A) is a target gene of estrogen and progesterone. It is over-expressed in breast cancer. However, little is known about the physiological function of TTC9A. The objectives of this study were to establish a Ttc9a knockout mouse model and to study the consequence of Ttc9a gene inactivation. The Ttc9a targeting vector(More)
Expression under the control of the mouse transferrin promoter of a transgene encoding a soluble secreted derivative of the ectodomain of the human insulin receptor in transgenic mice results in the accumulation of this high-affinity insulin-binding protein in the plasma. Alterations of glucose homeostasis are observed including postabsorptive hyperglycemia(More)
DNA analysis showed the presence of plasmids in all 29 strains of mesophilic lactic streptococci. Their number ranged from 1 to 9 and their molecular weight extended from 1.8 to 45 megadaltons (Md). Comparison of the plasmid profiles from 9 strains of Streptococcus lactis, 3 strains of S. lactis ssp. diacetylactis and from their Lac-,Prt-variants was(More)
This publication is the fruit of a collaborative effort between the Inter-American Association for Environmental Defense (AIDA), The Peruvian Society of Environmental Law (SPDA), and Earthjustice, with generous financial support from the John D. and Catherine T. MacArthur Foundation and the Conservation Food and Health Foundation. These organizations are(More)
The human insulin receptor (IR) is a transmembrane glycoprotein, whose cytoplasmic domain contains an insulin-activated protein-tyrosine kinase (EC 2.7.1.112). By the use of an appropriately engineered baculovirus expression vector, a soluble cytoplasmic derivative of this domain was expressed in the insect cell line Spodoptera frugiperda (Sf9). At 24 to 48(More)
Monoclonal antibody 5B4 recognizes a carboxy-terminal epitope common to large (approximately 180 kDa) and short (approximately 140 kDa) forms of neural cell adhesion molecules (NCAM-ld and NCAM-sd, respectively). The deduced primary sequence of rat brain 5B4/NCAM-ld predicts a large cytoplasmic domain (390 amino acids, Mr 39,284) of striking amino acid(More)
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