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Large yellow croaker (Larimichthys crocea) is an important economic fish in China and Eastern Asia. Because of the exhaustive fishing and overdense aquaculture, the wild population and the mariculture of the species are facing serious challenges on germplasm degeneration and susceptibility to infectious disease agents. However, a comprehensive transcriptome(More)
The genetic map of a species is essential for its whole genome assembly and can be applied to the mapping of important traits. In this study, we performed RNA-seq for a family of large yellow croakers (Larimichthys crocea) and constructed a high-density genetic map. In this map, 24 linkage groups comprised 3,448 polymorphic SNP markers. Approximately 72.4%(More)
Large yellow croaker, one of the most important economic marine fish in China, is mainly distributed in coastal regions of East Asia. Its wild stocks have been collapsed due to overfishing and habitat degradation. However, genetic analysis of the species for population conservation and molecular-aided selection has been largely hindered by insufficient(More)
Whole-genome single-nucleotide polymorphism (SNP) markers are valuable genetic resources for the association and conservation studies. Genome-wide SNP development in many teleost species are still challenging because of the genome complexity and the cost of re-sequencing. Genotyping-By-Sequencing (GBS) provided an efficient reduced representative method to(More)
A three-dimensional microfluidic origami glucose-air immuno-biofuel cell has been successfully demonstrated for the first time to implement self-powered, sensitive, and low-cost sandwich immunoassay for cancer markers based on the signal amplifications of a porous Au-paper electrode and Au nanoparticles attached to carbon nanotubes.
The Ti foil (0.1 mm thickness, 99.6 % purity, Goodfellow, England) was cleaned by using distilled water and acetone and dried off with N 2 gas. The bare Ti foil was anodized in an ethylene glycol solution containing NH 4 F (0.38 wt%) and H 2 O (1.79 wt%) and placed in a well-insulated bath for 3 h at 30 V and at 20 ℃ using a dc power supply (N5753A model,(More)
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