Pankhuri Singhal

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CRISPR-Cas systems have been broadly embraced as effective tools for genome engineering applications, with most studies to date utilizing the Streptococcus pyogenes Cas9. Here we characterize and manipulate the smaller, 1053 amino acid nuclease Staphylococcus aureus Cas9. We find that the S. aureus Cas9 recognizes an NNGRRT protospacer adjacent motif (PAM)(More)
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