Pamela M Donoghue

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The adaptive response of skeletal muscle fibres depends on a variety of biological factors including loading conditions and neuromuscular activity. An extreme type of atrophy-inducing change in contractile activity is represented by the physical disconnection between the motor nerve and its respective fibre unit. Since fibre type alterations have a striking(More)
Phospholemman (PLM, FXYD1), abundantly expressed in the heart, is the primary cardiac sarcolemmal substrate for PKA and PKC. Evidence supports the hypothesis that PLM is part of the cardiac Na-K pump complex and provides the link between kinase activity and pump modulation. PLM has also been proposed to modulate Na/Ca exchanger activity and may be involved(More)
The development of ECL-Plex CyDye-conjugated secondary antibodies allows the advancement of conventional Western blotting, opening up possibilities for highly sensitive and quantitative protein confirmation and identification. We report a novel proteomic method to simultaneously visualise the total protein profile as well as the specific immunodetection of(More)
Changes in cytoplasmic Ca 2+-levels regulate the contractile status of skeletal muscle fibres, whereby the finely tuned interplay between voltage sensors, Ca 2+-release channels, Ca 2+-binding proteins and Ca 2+-pumps mediates Ca 2+-cycling through the sarcoplasmic reticulum. Although the physical coupling between the α 1S-dihydropyridine receptor of the(More)
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