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AnAspergillus sp., isolated from a rubbish dump, produced 10.6 IU ml(-1) xylanase activity. Two xylanases were recognized and each was purified to homogeneity by two-stage chromatography on DEAE-and CM-Sepharose. Xylanase I had a pI of 7.2 and anM r of 26 kDa whereas xylanase II had a pI of 4.7 and anM r of 21 kDa. At 50°C, xylanase I was stable for 2.5 h(More)
A simultaneous concentration of enteroviruses, hepatitis E virus, and rotavirus from drinking water samples through a filtration column filled with granular activated carbon (GAC) was achieved. Urea-arginine phosphate buffer (UAPB) as an eluent at pH 9.0 was used for effective desorption and elution of viruses from GAC. Further concentration of viruses with(More)
The main objective of this study was to determine the applicability of the polymerase chain reaction (PCR) to detection of hepatitis E virus (HEV) in sewage treatment plants and establishment of the prevalence of hepatitis viral diseases in a population. Epidemics of HEV infection because of inadequate public sanitation have been reported in several(More)
We describe a membrane-filter-based urea-arginine phosphate buffer method for concentrating waterborne viruses from large volumes of water to microlitre volumes, and their subsequent detection by the polymerase chain reaction (PCR). The detection step involves the extraction of RNA, synthesis of complementary DNA, amplification by PCR of target DNA with(More)
Pseudomonas sp. strain PH1 was isolated from soil contaminated with pharmaceutical and dye industry waste. The isolate PH1 could use m-aminophenol as a sole source of carbon, nitrogen, and energy to support the growth. PH1 could degrade up to 0.32 mM m-aminophenol in 120 h, when provided as nitrogen source at 0.4 mM concentration with citrate (0.5 mM) as a(More)
Bacillus subtilis grown on glucose produced a de-emulsifier that broke down model oil-in-water emulsions. The deemulsification increased with contact time and the recovery of oil was 98% with 0.6 mg de-emulsifier/ml emulsion. GLC and i.r. analysis of the molecule extracted from cell-free broth demonstrated that the de-emulsifier was acetoin. It may be(More)
The widespread problem of contaminated water by enteric microorganisms necessitates the need to develop a rapid protocol to detect pathogens in water bodies. Usual methods like plating, biochemical tests and use of DNA probes are time consuming which is a limiting factor especially in epidemic situations. Moreover, some cells can exist in a virulent viable(More)
High temperature is one of the important stress factors that affect crops in tropical countries. Plants do evolve or adopt different mechanisms to overcome such stress for survival. It is an interesting subject and has attracted many researchers to work upon. Here, we studied the effect of salicylic acid (SA) on seedling growth and antioxidative defense(More)
Seedling growth and antioxidative defense system were studied in two spring maize (Zea mays L.) genotypes viz., CML 32 (relatively heat tolerant) and LM 11 (relatively heat susceptible), under heat stress (40 °C). Heat stress induced higher mobilization of biomass towards roots in CML32 genotype leading to increased root to shoot biomass ratio. The(More)