Pablo Hernán Strobl-Mazzulla

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The brain-type aromatase (CYP19A2) cDNA from pejerrey Odontesthes bonariensis was characterized. Its sequence differs from the ovarian-derived aromatase (CYP19A1) previously reported for the same species. The cDNA is 2305bp in length and the deduced protein comprises 501 amino-acids. The percentage of identity was higher when compared to other brain-derived(More)
Here, we explore whether silencing via promoter DNA methylation plays a role in neural versus neural crest cell lineage decisions. We show that DNA methyltransferase3A (DNMT3A) promotes neural crest specification by directly mediating repression of neural genes like Sox2 and Sox3. DNMT3A is expressed in the neural plate border, and its knockdown causes(More)
This work deals with a family of neuropeptides, gonadotropin-releasing hormone (GnRH), that play a key role in the development and maintenance of reproductive function in vertebrates. 2. Until now, a total of 16 GnRH structural variants have been isolated and characterized from vertebrate and protochordate nervous tissue. All vertebrate species already(More)
The neural crest is a migratory and multipotent cell population that plays a crucial role in many aspects of embryonic development. In all vertebrate embryos, these cells emerge from the dorsal neural tube then migrate long distances to different regions of the body, where they contribute to formation of many cell types and structures. These include much of(More)
Although estrogens exert many functions on vertebrate brains, there is little information on the relationship between brain aromatase and estrogen receptors. Here, we report the cloning and characterization of two estrogen receptors, alpha and beta, in pejerrey. Both receptors' mRNAs largely overlap and were predominantly expressed in the brain, pituitary,(More)
The development of gonadotropin-releasing hormone (GnRH) neurons was studied in relation to the sensitive period of thermolabile sex determination in the pejerrey Odontesthes bonariensis, an atherinid fish from South America. Fish were raised from hatching at three different temperatures: 17 degrees C (100% females), 24 degrees C (70% females), and 29(More)
The central nervous system of adult teleost fish is peculiar because of the following features: (1) the persistence of radial glial cells, (2) an important neurogenic activity and (3) a high aromatase expression by radial cells. In this study, the proliferative zones of the forebrain were described using bromodeoxyuridine (BrdU) treatment in the brain of(More)
Neural crest cells form within the neural tube and then undergo an epithelial to mesenchymal transition (EMT) to initiate migration to distant locations. The transcriptional repressor Snail2 has been implicated in neural crest EMT via an as of yet unknown mechanism. We report that the adaptor protein PHD12 is highly expressed before neural crest EMT. At(More)
The presence and distribution of growth hormone-releasing hormone (GHRH) were studied by immunocytochemistry in adult and developing pejerrey fish, Odontesthes bonariensis (Atheriniformes). A few perikarya and fibers with immunoreactivity to GHRH (ir-GHRH) were identified in the olfactory bulbs at hatching. One week later, scattered ir-GHRH cell bodies were(More)
In vertebrates, the inner ear arises from the otic placode, a thickened swathe of ectoderm that invaginates to form the otic vesicle. We report that histone demethylase KDM4B is dynamically expressed during early stages of chick inner ear formation. A loss of KDM4B results in defective invagination and striking morphological changes in the otic epithelium,(More)