PETER M. CANDIDO

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Histone methylation in developing trout testis has been observed in the diploid stem cells and primary spermatocytes, which actively synthesize DNA and histones. In spermatids, histone methylation is minimal and so probably plays no role in the replacement of histones by protamine which is characteristic of this cell type. No turnover of histone methyl(More)
The Caenorhabditis elegans ubiquitin-conjugating enzyme UBC-1 is distinct from other RAD6 homologues in possessing a C-terminal tail 40 amino acid residues long [Leggett, Jones and Candido (1995) DNA Cell Biol. 14, 883-891]. Such extensions from the core catalytic domain have been found in a subset of known conjugating enzymes, where they have been shown to(More)
Chromatin subunits or nucleosomes prepared by micrococcal nuclease digestion of nuclei from trout testis have been examined for the presence of in vivo modified histone species. Both monomers and multimers are labeled when testis cells are incubated in the presence of [14C]acetate, but the level of radioactivity in the monomer fraction is 10 to 20% higher(More)
The sequences of the first 25 residues of histone III, and the first 22 residues of histone Ib2, from trout testis have been determined on an automatic protein sequencer. The amino-terminal sequence of trout-testis histone III is identical to the corresponding region of calfthymus histone III, whereas the trout-testis histone IIb2 sequence differs from that(More)
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