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A new enzyme superfamily - the phosphopantetheinyl transferases.
TLDR
This work has identified a large family of proteins having 12-22 % similarity with ACPS, which are putative P-pant transferases, and found three of these proteins, E. coli EntD and o195, and subtilis Sfp, have been overproduced, purified and found to have P- pant transferase activity. Expand
Spx-dependent global transcriptional control is induced by thiol-specific oxidative stress in Bacillus subtilis
TLDR
It is proposed that Spx is an activator that mobilizes the operations necessary to reverse the effects of oxidative damage, but it also serves as a negative regulator that causes the postponement of developmental programs and energy-consuming growth-related functions while the cell copes with the period of stress. Expand
A regulatory protein that interferes with activator-stimulated transcription in bacteria
TLDR
The discovery of an “anti-α” factor Spx in Bacillus subtilis that blocks transcriptional activation by binding to the α-C-terminal domain, thereby interfering with the capacity of RNAP to respond to certain activator proteins. Expand
Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases.
The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidylExpand
Spatial variability overwhelms seasonal patterns in bacterioplankton communities across a river to ocean gradient
TLDR
Determining both the spatial and temporal variability of bacterioplankton communities provides a framework for modeling these communities across environmental gradients from river to deep ocean. Expand
Spx-RNA Polymerase Interaction and Global Transcriptional Control during Oxidative Stress
  • P. Zuber
  • Medicine, Biology
  • Journal of bacteriology
  • 1 April 2004
Spx is a unique RNA polymerase (RNAP)-binding protein that is highly conserved among low-G+C-content gram-positive bacteria and certain members of the Mollicutes . In the spore-forming bacteriumExpand
Redox‐sensitive transcriptional control by a thiol/disulphide switch in the global regulator, Spx
TLDR
It is shown here that Spx directly activates trxA and ‐B transcription by interacting with the RNA polymerase α‐subunit, but it does so only under an oxidized condition. Expand
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth
TLDR
Results show that nitrate respiration and fermentation of B. subtilis are governed by divergent regulatory pathways, and suggest that PDH is involved in most or all acetyl coenzyme A production in B.subilis. Expand
Management of oxidative stress in Bacillus.
  • P. Zuber
  • Biology, Medicine
  • Annual review of microbiology
  • 8 September 2009
The spore-forming bacterium and model prokaryotic genetic system, Bacillus subtilis, is extremely useful in the study of oxidative stress management through proteomic and genome-wide transcriptomicExpand
Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase.
TLDR
Analysis of the complex reveals interactions between three regions of "anti-alpha" Spx and helix alpha1 and the "261" determinant of alphaCTD, suggesting that Spx regulates transcription allosterically or through the redox-dependent creation or destruction of binding sites for additional components of the transcription machinery. Expand
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