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A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline
The data suggest that rasiRNAs protect the fly germline through a silencing mechanism distinct from both the miRNA and RNA interference pathways.
A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the let-7 Small Temporal RNA
In Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA, which regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals.
Small silencing RNAs: an expanding universe
Since the discovery in 1993 of the first small silencing RNA, a dizzying number of small RNA classes have been identified, including microRNAs (miRNAs), small interfering RNAs (siRNAs) and
RNAi Double-Stranded RNA Directs the ATP-Dependent Cleavage of mRNA at 21 to 23 Nucleotide Intervals
It is found that RNAi is ATP dependent yet uncoupled from mRNA translation, suggesting that the 21-23 nucleotide fragments from the dsRNA are guiding mRNA cleavage.
A Protein Sensor for siRNA Asymmetry
In Drosophila, R2D2 is both a protein sensor for siRNA thermodynamic asymmetry and a licensing factor for entry of authentic siRNAs into the RNAi pathway.
Passenger-Strand Cleavage Facilitates Assembly of siRNA into Ago2-Containing RNAi Enzyme Complexes
It is shown that Ago2 instead directly receives the double-stranded siRNA from the RISC assembly machinery, and then cleaves the siRNA passenger strand, thereby liberating the single-Stranded guide.
A microRNA in a Multiple-Turnover RNAi Enzyme Complex
It is shown that, in human cell extracts, the miRNA let-7 naturally enters the RNAi pathway, which suggests that only the degree of complementarity between a miRNA and its RNA target determines its function.