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The Grainyhead-like epithelial transactivator Get-1/Grhl3 regulates epidermal terminal differentiation and interacts functionally with LMO4.
Findings indicate that the Get-1 and LMO4 genes interact functionally to regulate epidermal terminal differentiation, which affects multiple genes linked to terminal differentiation and barrier function. Expand
The composition of the ceramides from human stratum corneum and from comedones.
Human epidermal surface lipids were collected by an ethanol wash and the ceramides were quantified by thin-layer chromatography-photodensitometry, supporting the hypothesis that a localized insufficiency of linoleic acid in the follicular epithelium is an etiologic factor in comedogenesis. Expand
Molecular models of the intercellular lipid lamellae in mammalian stratum corneum.
Intercellular lipid lamellae in the stratum corneum constitute the barrier to water diffusion and may also play a role in cohesion between corneocytes. The lamellae arise from stacks of lamellarExpand
Ceramide and cholesterol composition of the skin of patients with atopic dermatitis.
The data confirm the view that impaired metabolism of ceramides may be the cause of dry skin and impaired barrier function in atopic dermatitis and suggest that a decrease in ceramide in the stratum corneum is involved in barrier impairment in atopy dermatitis skin. Expand
Presence of intact intercellular lipid lamellae in the upper layers of the stratum corneum.
Stacking of lamellae filling the intercellular spaces in the uppermost layers of the stratum corneum is demonstrated and suggests that the lipid bilayer adjacent to the corneocyte cell envelope may be assembled from lipids not derived from MCGs. Expand
The human epidermis models EpiSkin, SkinEthic and EpiDerm: an evaluation of morphology and their suitability for testing phototoxicity, irritancy, corrosivity, and substance transport.
First results indicate their suitability for transport experiments of drugs and other xenobiotics across skin, but the barrier function of these reconstructed human epidermis models appears to be much less developed compared to native skin. Expand
Composition and morphology of epidermal cyst lipids.
The contents of epidermal cysts were used as a source of desquamated human keratinocytes uncontaminated by sebaceous, subcutaneous, or bacterial lipids and indicate that the degradation of intercellular lipid lamellae is not required for desquamation. Expand
Covalently bound ω-hydroxyacylsphingosine in the stratum corneum
Pig epidermis was heat separated, and the stratum corneum was isolated after trypsinization. Exhaustive extraction of the stratum corneum fraction with chloroform/methanol mixtures yielded 14.7%Expand
Ceramides of pig epidermis: structure determination.
Seven distinguishable groups of ceramides have been isolated from pig epidermis and identified and apparently form a large proportion of the intercellular lipid lamellae in the epidermal stratum corneum, which are thought to constitute the barrier to water loss. Expand
Covalently bound lipids of human stratum corneum.
It is demonstrated that human stratum corneum contains covalently bound lipids accounting for 1.4% of the dry weight of the tissue, and it is suggested that the principal function of the covalent bound lipid is the formation of a lipid envelope on the outer surface of the keratinized cells. Expand