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Crystal structure and functional mapping of human ASMT, the last enzyme of the melatonin synthesis pathway
TLDR
The X‐ray crystal structure of human N‐acetyl serotonin methyltransferase (ASMT), the last enzyme of the melatonin biosynthesis pathway, is presented and mapping of the variants on to the 3‐dimensional structure clarifies why some are harmful and provides a structural basis for understanding melatonin deficiency in humans.
Purification and characterization of cysteine-S-conjugate N-acetyltransferase from pig kidney.
TLDR
The purification and more detailed characterization of this enzyme catalysing the final reaction of mercapturic acid biosynthesis, and thus playing a crucial role in the detoxicating metabolism of many xenobiotics, is described.
A nonradioactive assay for microsomal cysteine-S-conjugate N-acetyltransferase activity by high-pressure liquid chromatography.
TLDR
A new nonradioactive assay is developed that is sensitive, less costly, safer, less time-consuming, and less laborious than radioactive assays for cysteine-S-conjugate N-acetyltransferase activity.
The histochemical application of dansylhydrazine as a fluorescent labeling reagent for sialic acid in cellular glycoconjugates
TLDR
A new method for the fluorescent staining of sialic acid—containing glycoconjugates in fixed tissues is described, which gives superior resolution in comparison to the standard periodate Schiff procedure for cellular carbohydrates.
Structure and function of the Leptospira interrogans peroxide stress regulator (PerR), an atypical PerR devoid of a structural metal-binding site
TLDR
This study demonstrates that structural metal binding is not compulsory for PerR dimeric assembly and for regulating peroxide stress.
A novel Plasmodium-specific prodomain fold regulates the malaria drug target SUB1 subtilase.
TLDR
The SUB1 structure not only provides a valuable platform to develop new anti-malarial candidates against this promising drug target, but also defines the Plasmodium-specific 'belt' domain as a key calcium-dependent regulator of SUB1 during parasite egress from host cells.
Structural and functional features of Crl proteins and identification of conserved surface residues required for interaction with the RpoS/σS subunit of RNA polymerase.
TLDR
Examination of the X-ray crystal structure of the Proteus mirabilis Crl protein and a structural model for Salmonella Typhimurium Crl (CrlSTM) suggests that Crl family members share a common mechanism of σS binding in which the flexible arms of Crl might play a dynamic role.
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