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Rapid and sequential movement of individual chromosomal loci to specific subcellular locations during bacterial DNA replication.
Time-lapse microscopy of the location of the chromosomal origin and 10 selected loci in the origin-proximalhalf of the chromosome showed that during DNA replication, as the replisome sequentially copies each locus, the newly replicated DNA segments are moved in chronological order to their final subcellular destination in the nascent half of the predivisional cell. Expand
Identification of a localization factor for the polar positioning of bacterial structural and regulatory proteins
A protein is identified, PodJ, that provides the positional information for the polar localization of both PleC and CpaE, and stimulates the response signaled by a two-component system in Caulobacter. Expand
Oscillating Global Regulators Control the Genetic Circuit Driving a Bacterial Cell Cycle
A newly identified cell-cycle master regulator protein, GcrA, together with the CtrA master regulator, are key components of a genetic circuit that drives cell-cycle progression and asymmetric polarExpand
Bacterial Birth Scar Proteins Mark Future Flagellum Assembly Site
TipN and TipF establish a link between bacterial cytokinesis and polar asymmetry, demonstrating that division does indeed leave a positional mark in its wake to direct the biogenesis of a polar organelle. Expand
A membrane metalloprotease participates in the sequential degradation of a Caulobacter polarity determinant
The sequential degradation of PodJ appears to involve regulated intramembrane proteolysis (Rip) by MmpA, and it is determined that mmpA and yaeL can complement each other in C. crescentus and E. coli, indicating functional conservation. Expand
A connection between stress and development in the multicellular prokaryote Streptomyces coelicolor A3(2)
These data show that expression of σH is subject to temporal and spatial regulation during colony development, that this tissue‐specific regulation is mediated directly by the developmental transcription factor BldD and suggest that stress and developmental programmes may be intimately connected in Streptomyces morphogenesis. Expand
Coupling prokaryotic cell fate and division control with a bifunctional and oscillating oxidoreductase homolog.
A bifunctional NAD(H)-binding regulator (KidO) is identified that integrates cell-fate signaling with cytokinesis in the bacterium Caulobacter crescentus and exploits the enzymatic fold of an ancestral oxidoreductase potentially to coordinate cellular or developmental activities with the availability of the metabolic currency, NAD(P)H. Expand
A dynamically localized histidine kinase controls the asymmetric distribution of polar pili proteins
It is demonstrated that the PleC histidine kinase, a two‐component signal transduction protein shown previously to localize to the piliated cell pole before and during pilus assembly, controls the accumulation of the pilin subunit, PilA. Expand
DivL Performs Critical Cell Cycle Functions in Caulobacter crescentus Independent of Kinase Activity
The results show that DivL controls two key cell cycle regulators, CtrA and DivK, and that phosphoryl transfer is not DivL's essential cellular activity. Expand
The dynamic interplay between a cell fate determinant and a lysozyme homolog drives the asymmetric division cycle of Caulobacter crescentus.
The dynamic interplay between SpmX and DivK is at the heart of the molecular circuitry that sustains the Caulobacter developmental cycle. Expand