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Characterization of a Murine Monoclonal Antibody toCryptococcus neoformans Polysaccharide That Is a Candidate for Human Therapeutic Studies
The results show that both the variable and constant regions of MAb 18B7 are biologically functional and support the use of this MAb in human therapeutic trials.
Human-liver cytochromes P-450 expressed in yeast as tools for reactive-metabolite formation studies. Oxidative activation of tienilic acid by cytochromes P-450 2C9 and 2C10.
The results suggest the important role of P450 2C9 in the oxidative metabolism of tienilic acid in human liver and indicate that the 5-hydroxylation reaction could be a useful marker for P450 1C9 activity and underline the interest of human liver P450s expressed in yeast as tools for studying the formation of reactive metabolites.
Thiophene sulfoxides as reactive metabolites: formation upon microsomal oxidation of a 3-aroylthiophene and fate in the presence of nucleophiles in vitro and in vivo.
Interestingly, after treatment of rats with 1, metabolites 8a and 8b could be detected in urine, indicating that the successive reactions, that were observed in vitro after microsomal oxidation of 1 in the presence of a thiol-containing trapping agent, also occur in vivo, glutathione acting as a nucleophile in that case.
Peptide libraries define the fine specificity of anti-polysaccharide antibodies to Cryptococcus neoformans.
It is shown that peptide mimotopes for a complex microbial polysaccharide can be identified by screening phage peptide libraries and demonstrates the usefulness of such peptides in analyzing closely related interactive sites of proteins in general and of antibodies in particular.
The three-dimensional structures of a polysaccharide binding antibody to Cryptococcus neoformans and its complex with a peptide from a phage display library: implications for the identification of
The three-dimensional structure of 2H1, a protective monoclonal antibody to Cryptococcus neoformans, has been solved at 2.4 A resolution, in both its unbound form and in complex with the 12 amino
Specificity of in vitro covalent binding of tienilic acid metabolites to human liver microsomes in relationship to the type of hepatotoxicity: comparison with two directly hepatotoxic drugs.
There may exist a relationship between the specificity of covalent binding and the type of hepatotoxicity, and the target of tienilic acid-reactive metabolites was identified as cytochrome P450 2C9, and experiments with yeast expressing active isolated P450s showed that P4502C9 was responsible for tienic acid- reactive metabolite(s) production.
High Affinity Mimotope of the Polysaccharide Capsule of Cryptococcus neoformans Identified from an Evolutionary Phage Peptide Library1
Efforts to improve this vaccine are reported by screening a sublibrary with six random amino acids added to either end of the PA1 motif to identify higher affinity peptides.
Screening phage display libraries for organ-specific vascular immunotargeting in vivo.
A strategy to identify probes and their cognate antigens for targeting vascular endothelia of specific organs in vivo by differentially screen phage libraries to select organ-targeting antibodies by using luminal endothelial cell plasma membranes isolated directly from tissue and highly enriched in natively expressed proteins exposed to the bloodstream.