• Publications
  • Influence
Understanding the relationship between the primary structure of proteins and its propensity to be soluble on overexpression in Escherichia coli
Solubility of proteins on overexpression in Escherichia coli is a manifestation of the net effect of several sequence‐dependent and sequence‐independent factors. This study aims to delineate theExpand
  • 142
  • 4
  • PDF
Identification of linkage-specific sequence motifs in sialyltransferases.
Eukaryotic sialyltransferases (SiaTs) comprise a superfamily of enzymes catalyzing the transfer of sialic acid (Sia) from a common donor substrate to various acceptor substrates in differentExpand
  • 57
  • 4
Identification of common structural features of binding sites in galactose‐specific proteins
Galactose‐binding proteins characterize an important subgroup of sugar‐binding proteins that are involved in a variety of biological processes. Structural studies have shown that the Gal‐specificExpand
  • 70
  • 3
Griseofulvin stabilizes microtubule dynamics, activates p53 and inhibits the proliferation of MCF-7 cells synergistically with vinblastine
BackgroundGriseofulvin, an antifungal drug, has recently been shown to inhibit proliferation of various types of cancer cells and to inhibit tumor growth in athymic mice. Due to its low toxicity,Expand
  • 78
  • 3
A support vector machine-based method for predicting the propensity of a protein to be soluble or to form inclusion body on overexpression in Escherichia coli
A support vector machine-based classifier to predict the solubility status of proteins on overexpression in E.coli based on the sequencebased features of these proteins. Expand
  • 95
  • 3
  • PDF
Mutational analysis of the Golgi retention signal of bovine beta-1,4-galactosyltransferase.
To examine the role of the NH2-terminal region of the 402-residue-long beta-1,4-galactosyltransferase (beta-1,4-GT), a series of mutants and chimeric cDNA were constructed by polymerase chainExpand
  • 114
  • 2
  • PDF
Crystal structure of beta1,4-galactosyltransferase complex with UDP-Gal reveals an oligosaccharide acceptor binding site.
The crystal structure of the catalytic domain of bovine beta1,4-galactosyltransferase (Gal-T1) co-crystallized with UDP-Gal and MnCl(2) has been solved at 2.8 A resolution. The structure not onlyExpand
  • 100
  • 2
Comparative genomics analysis of completely sequenced microbial genomes reveals the ubiquity of N-linked glycosylation in prokaryotes.
Glycosylation of proteins in prokaryotes has been known for the last few decades. Glycan structures and/or the glycosylation pathways have been experimentally characterized in only a small number ofExpand
  • 12
  • 2
Characterization of symmetric and asymmetric lipid bilayers composed of varying concentrations of ganglioside GM1 and DPPC.
Gangliosides are a group of structurally diverse, sialic acid containing glycosphingolipids embedded into the membrane via their hydrophobic ceramide moiety. To gain atomic level insights into theExpand
  • 31
  • 1