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RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension
An RNA molecule is described that catalyzes the type of polymerization needed for RNA replication, which uses nucleoside triphosphates and the coding information of an RNA template to extend an RNA primer by the successive addition of up to 14 nucleotides.
Comparative analysis of the small RNA transcriptomes of Pinus contorta and Oryza sativa.
The conservation of microRNAs and other small RNAs between the conifers and the angiosperms indicates that important RNA silencing processes were highly developed in the earliest spermatophytes.
RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking.
By inserting RNA Mango into a stem loop of the bacterial 6S RNA and biotinylating the fluorophore, it is demonstrated that the aptamer can be used to simultaneously fluorescently label and purify biologically important RNAs.
Extensive 3' modification of plant small RNAs is modulated by helper component-proteinase expression.
- H. Ebhardt, E. P. Thi, Ming-Bo Wang, P. Unrau
- BiologyProceedings of the National Academy of Sciences…
- 20 September 2005
It is shown that a large fraction of all silencing-associated sm RNAs in tobacco are modified; this modification occurs on the 2' hydroxyl of the terminal ribose and significantly reduces the cloning efficiency of these modified smRNAs.
Selection of an improved RNA polymerase ribozyme with superior extension and fidelity.
The isolation of an RNA polymerase ribozyme called B6.61 that exhibits superior extension and fidelity relative to its progenitor, the Round-18 polymerase, demonstrates the feasibility of evolving an artificial RNA replicase Ribozyme in the foreseeable future.
RNA-catalysed nucleotide synthesis
The finding that RNA can catalyse this type of reaction, which is modelled after pyrimidine synthesis in contemporary metabolism, supports the idea of an RNA world that included nucleotide synthesis and other metabolic pathways mediated by ribozymes.
Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells
Three new high-affinity RNA Mango fluorogenic aptamers with improved fluorescent properties for both fixed and live-cell imaging are isolated by using competitive ligand binding microfluidic selection.
Conifers have a unique small RNA silencing signature.
It is found that conifers, which radiated from the other seed-bearing plants approximately 260 million years ago, fail to produce significant amounts of 24-nucleotide RNAs that are known to guide DNA methylation and heterochromatin formation in angiosperms, Instead, they synthesize a diverse population of small RNas that are exactly 21-nt long.
Small RNAs derived from tRNAs and rRNAs are highly enriched in exosomes from both old and new world Leishmania providing evidence for conserved exosomal RNA Packaging
- U. Lambertz, M. Oviedo Ovando, Elton J R Vasconcelos, P. Unrau, P. Myler, N. Reiner
- BiologyBMC Genomics
- 5 March 2015
It is shown that leishmania exosomes are selectively and specifically enriched in small RNAs derived almost exclusively from non-coding RNAs, which open up a new avenue of research on non-canonical, small RNA pathways in this trypanosomatid.